EVIDENCE FOR AMINO-ACID SEQUENCE OF CRITHIDIA-FASCICULATA CYTOCHROME C555
EVIDENCE FOR AMINO-ACID SEQUENCE OF CRITHIDIA-FASCICULATA CYTOCHROME C555
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DOI：
10.1111/j.1432-1033.1975.tb02298.x
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
PETTIGREW, GW
中科院分区：
文献类型：
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作者：
HILL, GC;PETTIGREW, GW
提供的证据表明来自Critithia fasciculata的细胞色素c555的氨基酸序列与来自Critithia oncopelti的细胞色素c557的氨基酸序列在16个位置上不同。通过丹酰-埃德曼降解、羧肽酶消化或考虑胰蛋白酶的特异性鉴定了 101 个残基，其中 13 个残基被发现与 C 不同。肿瘤细胞色素c557。在胰蛋白酶肽的氨基酸组成中发现的其余11个残基根据与细胞色素c557的同源性进行比对，并提出了三个进一步的差异。鉴于这些生物体的形态和生化相似性，总共十六个氨基酸差异是令人惊讶的，并说明了形态简单的生物体的分类学问题。在两种细胞色素中，只有一个半胱氨酸残基参与蛋白质与辅基的附着。
Evidence is presented which indicates that the amino acid sequence of cytochromec555fromCrithidia fasciculatadiffers at sixteen positions from that of cytochromec557fromCrithidia oncopelti. 101 residues were identified by dansyl‐Edman degradation, carboxypeptidase digestion or considerations of the specificity of trypsin and of these, thirteen were found to be different fromC. oncopelticytochromec557. The remaining 11 residues found in the amino acid compositions of the tryptic peptides were aligned on the basis of homology with cytochromec557and three further differences are proposed.The total of sixteen amino acid differences is surprising in view of the morphological and biochemical similarities of these organisms, and illustrates the problems of taxonomy of morphologically simple organisms. In both cytochromes only one cysteine residue is involved in the attachment of the protein to the prosthetic group.