MicroRNA miR-155 is a biomarker of early pancreatic neoplasia
MicroRNA miR-155 is a biomarker of early pancreatic neoplasia
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DOI：
10.4161/cbt.8.4.7338
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发表时间：
2009-02-15
影响因子：
3.6
通讯作者：
Maitra, Anirban
中科院分区：
文献类型：
--
作者：
Habbe, Nils;Koorstra, Jan-Bart M.;Maitra, Anirban
背景：导管内乳头状粘液瘤（IPMNs）是胰腺癌的非侵袭性前驱病变。microRNAs （miRNAs）的错误表达在胰腺腺癌中很常见。相比之下，胰腺癌前驱病变中的miRNA异常尚未被证实。实验设计：采用定量反转录PCR （qRT-PCR）技术，在15例非侵入性ipmn中评估胰腺癌中上调的12种mirna的相对表达水平。通过锁定核酸原位杂交（LNA-ISH）在64个档案ipmn中评估两个显著过表达的mirna - mir -155和mir -21。在10例手术切除的ipmn患者和5例非肿瘤性胰胆管疾病患者（“疾病对照”）的胰液样本中，也评估了miR-155和miR-21的表达。结果：通过qRT-PCR，在15个ipmn中观察到12个mirna中的10个与匹配对照组相比显着过表达(p
Background: Intraductal papillary mucinous neoplasms (IPMNs) are non-invasive precursor lesions of pancreatic cancer. Misexpression of microRNAs (miRNAs) is commonly observed in pancreatic adenocarcinoma. In contrast, miRNA abnormalities in pancreatic cancer precursor lesions have not been documented.Experimental design: Relative expression levels of a panel of twelve miRNAs upregulated in pancreatic cancers were assessed in 15 non-invasive IPMNs, using quantitative reverse transcription PCR (qRT-PCR). Two significantly overexpressed miRNAs-miR-155 and miR-21-were evaluated by locked nucleic acid in situ hybridization (LNA-ISH) in a panel of 64 archival IPMNs. The expression of miR-155 and miR-21 was also evaluated in pancreatic juice samples obtained from ten patients with surgically resected IPMNs and five patients with non-neoplastic pancreatobiliary disorders ("disease controls").Results: Significant overexpression by qRT-PCR of ten of the twelve miRNAs was observed in the 15 IPMNs versus matched controls (p