A Novel High Mechanical Property PLGA Composite Matrix Loaded with Nanodiamond-Phospholipid Compound for Bone Tissue Engineering.
A Novel High Mechanical Property PLGA Composite Matrix Loaded with Nanodiamond-Phospholipid Compound for Bone Tissue Engineering.
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DOI：
10.1021/acsami.5b09394
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发表时间：
2016-01
影响因子：
9.5
通讯作者：
Fanrou Zhang;Qing-xin Song;Xuan Huang;Feng-ning Li;Kun Wang;Yi-xing Tang;Cang-long Hou;Hongxing Shen
中科院分区：
文献类型：
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作者：
Fanrou Zhang;Qing-xin Song;Xuan Huang;Feng-ning Li;Kun Wang;Yi-xing Tang;Cang-long Hou;Hongxing Shen
将纳米金刚石磷脂复合物（NDPC）负载于可生物降解的聚乳酸-羟基乙酸共聚物（PLGA）中，通过物理混合制备了一种潜在的骨组织工程材料。基于纳米金刚石的疏水作用和物理吸附作用，利用磷脂对纳米金刚石的亲水表面进行改性，使其具有两亲性，形成典型的核壳结构。优化ND-磷脂重量比以产生样品NDPC 50（即，ND-磷脂重量比为100：50），并且NDPC 50能够以高达20重量%分散在PLGA基质中。与纯PLGA基质相比，引入10重量%的NDPC（即，样品NDPC 50-PF 10）导致材料的机械和表面性能显著改善，包括水接触角从80 °降低到55°，杨氏模量增加约100%，硬度增加约550%，因此与人皮质骨非常相似。作为一种新型的支持人成骨细胞（hFOB1.19）生长的基质，NDPC 50-PF与不同数量的NDPC 50表现出对细胞增殖和成骨分化没有负面影响。此外，我们重点研究了NDPC-PF植入小鼠体内8周的行为，发现NDPC-PF诱导了可接受的免疫应答，并可以减少PLGA基质的快速生物降解。我们的研究结果代表了第一次在体内研究ND（或NDPC）作为纳米填料的聚合物基质骨组织工程。高的机械性能，良好的体外和体内生物相容性，和增加的矿化能力表明，可生物降解的PLGA复合材料基质加载NDPC可能是有用的各种生物医学应用，特别是骨组织工程。
A potential bone tissue engineering material was produced from a biodegradable polymer, poly(lactic-co-glycolic acid) (PLGA), loaded with nanodiamond phospholipid compound (NDPC) via physical mixing. On the basis of hydrophobic effects and physical absorption, we modified the original hydrophilic surface of the nanodiamond (NDs) with phospholipids to be amphipathic, forming a typical core-shell structure. The ND-phospholipid weight ratio was optimized to generate sample NDPC50 (i.e., ND-phospholipid weight ratio of 100:50), and NDPC50 was able to be dispersed in a PLGA matrix at up to 20 wt %. Compared to a pure PLGA matrix, the introduction of 10 wt % of NDPC (i.e., sample NDPC50-PF10) resulted in a significant improvement in the material's mechanical and surface properties, including a decrease in the water contact angle from 80 to 55°, an approximately 100% increase in the Young's modulus, and an approximate 550% increase in hardness, thus closely resembling that of human cortical bone. As a novel matrix supporting human osteoblast (hFOB1.19) growth, NDPC50-PFs with different amounts of NDPC50 demonstrated no negative effects on cell proliferation and osteogenic differentiation. Furthermore, we focused on the behaviors of NDPC-PFs implanted into mice for 8 weeks and found that NDPC-PFs induced acceptable immune response and can reduce the rapid biodegradation of PLGA matrix. Our results represent the first in vivo research on ND (or NDPC) as nanofillers in a polymer matrix for bone tissue engineering. The high mechanical properties, good in vitro and in vivo biocompatibility, and increased mineralization capability suggest that biodegradable PLGA composite matrices loaded with NDPC may potentially be useful for a variety of biomedical applications, especially bone tissue engineering.