DISTRIBUTION OF NERVE GROWTH-FACTOR IN THE MALE SEX-ORGANS OF MAMMALS
DISTRIBUTION OF NERVE GROWTH-FACTOR IN THE MALE SEX-ORGANS OF MAMMALS
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DOI：
10.1111/j.1471-4159.1980.tb09663.x
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发表时间：
1980-01-01
影响因子：
4.7
通讯作者：
THOENEN, H
中科院分区：
文献类型：
--
作者：
HARPER, GP;THOENEN, H
The nerve growth factor (NGF) content of male sex organs of the mouse, rat, guinea pig, hamster, rabbit, human and bull was investigated using both a biological assay and a 2-site radioimmunoassay. The prostate glands of the rabbit and bull contained moderate levels of NGF, being lower than the concentrations found in the guinea pig prostate and mouse submaxillary glands. The sex organs investigated in the mouse, rat, hamster and human contained no detectable NGF activity. Genital organs, other than the prostate glands, in the guinea pig and rabbit were also devoid of NGF. NGF in the rabbit and bull are immunologically related to those found in the submaxillary glands of the mouse and the prostate glands of the guinea pig, but immunodiffusion and radioimmunoassay experiments show that there are also clear differences between the NGF. The use of a two-site radioimmunoassay, based on purified antibodies against mouse submaxillary gland NGF, for the determination of NGF levels in species other than the mouse, is described. It is essential during such applications to keep in mind that NGF from different species are sufficiently distinct that only part of the antibody population (raised against mouse NGF) is capable of recognizing NGF from species other than the mouse. The results of radioimmunoassay and biological assay determinations are in reasonable agreement, if corrections for this feature are made.
