Gapless assembly of maize chromosomes using long-read technologies
Gapless assembly of maize chromosomes using long-read technologies
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DOI：
10.1186/s13059-020-02029-9
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发表时间：
2020-05-20
期刊：
影响因子：
12.3
通讯作者：
Dawe, R. Kelly
中科院分区：
文献类型：
--
作者：
Liu, Jianing;Seetharam, Arun S.;Dawe, R. Kelly
创造复杂基因组的无缝隙端粒到端粒组合是基因组学的终极挑战之一。我们使用两个独立的组件和一个基于光学图谱的合并管道来产生一个由63个重叠群和一个162Mb的重叠群N50组成的玉米基因组(B73-AB10)。该基因组包括3号染色体(236Mb)和9号染色体(162Mb)的无缝组合，以及AB10减数分裂驱动单倍型的53Mb。数据还揭示了7个着丝粒和5个异色旋钮的内部结构，表明主要的串联重复阵列(CentC、Knob180和TR-1)是不连续的，经常夹杂着逆转录元件。
Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 Mb. This genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), and 53 Mb of the Ab10 meiotic drive haplotype. The data also reveal the internal structure of seven centromeres and five heterochromatic knobs, showing that the major tandem repeat arrays (CentC, knob180, and TR-1) are discontinuous and frequently interspersed with retroelements.