Process Simplification and Structure Design of Parallelized Microslit Isolator for Physical Property-based Capture of Tumor Cells
Process Simplification and Structure Design of Parallelized Microslit Isolator for Physical Property-based Capture of Tumor Cells
复制标题
基于物理特性捕获肿瘤细胞的并行微缝隔离器的工艺简化和结构设计
DOI：
10.1039/d2an00052k
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发表时间：
2022
期刊：
影响因子：
4.2
通讯作者：
and Minoru Seki
中科院分区：
文献类型：
--
作者：
Natsumi Shimmyo;Mayu Furuhata;Masumi Yamada*;Rie Utoh;and Minoru Seki
已经进行了许多尝试来开发有效的系统以从血液样品中纯化痕量的循环肿瘤细胞（CTC）。然而，目前的技术受到器件制造、系统设计和工艺可操作性的复杂性的限制。在这里，我们描述了一个简单的，可扩展的，和高效的方法来物理捕获CTC使用合理设计的微流体隔离器与微缝通道阵列。宽而薄的微缝通道具有几微米的深度，选择性地捕获CTC，CTC比其他血细胞更大且更不易变形，同时允许其他血细胞流过。我们详细研究了微通道的几何形状和操作参数对几种类型的培养的肿瘤细胞的捕获效率和选择性的影响，这些肿瘤细胞作为CTC模型掺入血液样品中。此外，捕获的细胞的原位捕获后染色被证明用于研究该系统对临床癌症诊断的适用性。所提出的方法操作简单，但在捕获特定细胞方面显着有效，因此它可能在实施基于细胞物理学的CTC分离技术用于癌症液体活检方面具有很大的潜力。
Numerous attempts have been made to develop efficient systems to purify trace amounts of circulating tumor cells (CTCs) from blood samples. However, current technologies are limited by complexities in device fabrication, system design, and process operability. Here we describe a facile, scalable, and highly efficient approach to physically capturing CTCs using a rationally designed microfluidic isolator with an array of microslit channels. The wide but thin microslit channels with a depth of several micrometers selectively capture CTCs, which are larger and less deformable than other blood cells, while allowing other blood cells to just flow through. We investigated in detail the effects of the microchannel geometry and operating parameters on the capture efficiency and selectivity of several types of cultured tumor cells spiked in blood samples as the CTC model. Additionally, in situ post-capture staining of the captured cells was demonstrated to investigate the system's applicability to clinical cancer diagnosis. The presented approach is simple in operation but significantly effective in capturing specific cells and hence it may have great potential in implementating cell physics-based CTC isolation techniques for cancer liquid biopsy.