Diversity of Oligotrichia and Choreotrichia Ciliates in Coastal Marine Sediments and in Overlying Plankton
Diversity of Oligotrichia and Choreotrichia Ciliates in Coastal Marine Sediments and in Overlying Plankton
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DOI：
10.1128/aem.01604-09
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发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Katz, Laura A.
中科院分区：
文献类型：
--
作者：
Doherty, Mary;Tamura, Maiko;Katz, Laura A.
阐明底栖纤毛虫群落与浮游生物之间的关系是了解海洋系统纤毛虫多样性的关键。虽然许多谱系的数据是稀疏的，至少有一些占主导地位的海洋纤毛虫分支寡毛和Choreotrichia的成员可以在浮游生物和底栖生物中找到，在后者无论是作为囊肿或活跃的形式。在这项研究中，我们开发了一种分子方法来解决浮游生物中的纤毛虫的多样性之间的关系，并在相同的位置下的底栖生物。比较了新英格兰海岸沿着三个地点的浮游生物和沉积物样本，并分析了额外的子样本以评估方法的重现性。我们发现，沉积物和浮游生物的子样本不同的鲁棒性重复子采样。沉积物子样本（即，1克等分试样从一个单一的类似20克的样本）给多样性的变量估计，而浮游生物子样本产生一致的结果。这些结果表明，需要进行更多的研究，以确定海洋沉积物中多样性变化的空间尺度。聚类的系统发育类型表明，底栖组合的寡毛和choreotrichs似乎更像那些从空间上遥远的底栖生物群落比纤毛虫群落采样在他们上面的水。
Elucidating the relationship between ciliate communities in the benthos and the plankton is critical to understanding ciliate diversity in marine systems. Although data for many lineages are sparse, at least some members of the dominant marine ciliate clades Oligotrichia and Choreotrichia can be found in both plankton and benthos, in the latter either as cysts or active forms. In this study, we developed a molecular approach to address the relationship between the diversity of ciliates in the plankton and those of the underlying benthos in the same locations. Samples from plankton and sediments were compared across three sites along the New England coast, and additional subsamples were analyzed to assess reproducibility of methods. We found that sediment and plankton subsamples differed in their robustness to repeated subsampling. Sediment subsamples (i.e., 1-g aliquots from a single similar to 20-g sample) gave variable estimates of diversity, while plankton subsamples produced consistent results. These results indicate the need for additional study to determine the spatial scale over which diversity varies in marine sediments. Clustering of phylogenetic types indicates that benthic assemblages of oligotrichs and choreotrichs appear to be more like those from spatially remote benthic communities than the ciliate communities sampled in the water above them.