Concurrent recordings of bladder afferents from multiple nerves using a microfabricated PDMS microchannel electrode array
Concurrent recordings of bladder afferents from multiple nerves using a microfabricated PDMS microchannel electrode array
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DOI：
10.1039/c2lc21277c
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Lacour, Stephanie P.
中科院分区：
文献类型：
--
作者：
Delivopoulos, Evangelos;Chew, Daniel J.;Lacour, Stephanie P.
在本文中，我们提出了一个顺应性的神经接口，旨在记录膀胱传入活动。我们使用多层硅橡胶和薄金属开发了植入物的微加工工艺，以便将金微电极阵列嵌入四个平行的聚二甲基硅氧烷（PDMS）微通道（5 mm长，100 mm宽，100 mm深）中。在1 kHz的电极阻抗进行了优化，使用反应离子蚀刻（RIE）的步骤，这增加了电极表面的孔隙率。电极在37 ℃的磷酸盐缓冲盐水（PBS）中浸泡3个月后没有劣化。由于PDMS上金属膜的独特微观形貌，电极非常柔顺，可以承受植入过程中的处理（扭曲和弯曲），而不会发生电气故障。将该装置急性移植到麻醉大鼠中，并将L6和S1根的背侧分支的股线通过手术梳理并插入盐水浸泡下的三个微通道中，以允许在急性环境中同时进行体内记录。我们利用三极电极配置来保持低背景噪声并提高信噪比。该装置可以区分与增加膀胱充盈和收缩相关的两种类型的传入神经活动。据我们所知，这是第一个报告的多通道记录膀胱传入活动。
In this paper we present a compliant neural interface designed to record bladder afferent activity. We developed the implant's microfabrication process using multiple layers of silicone rubber and thin metal so that a gold microelectrode array is embedded within four parallel polydimethylsiloxane (PDMS) microchannels (5 mm long, 100 mm wide, 100 mm deep). Electrode impedance at 1 kHz was optimized using a reactive ion etching (RIE) step, which increased the porosity of the electrode surface. The electrodes did not deteriorate after a 3 month immersion in phosphate buffered saline (PBS) at 37 degrees C. Due to the unique microscopic topography of the metal film on PDMS, the electrodes are extremely compliant and can withstand handling during implantation (twisting and bending) without electrical failure. The device was transplanted acutely to anaesthetized rats, and strands of the dorsal branch of roots L6 and S1 were surgically teased and inserted in three microchannels under saline immersion to allow for simultaneous in vivo recordings in an acute setting. We utilized a tripole electrode configuration to maintain background noise low and improve the signal to noise ratio. The device could distinguish two types of afferent nerve activity related to increasing bladder filling and contraction. To our knowledge, this is the first report of multichannel recordings of bladder afferent activity.