Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows
Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows
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DOI：
10.1016/s0093-691x(00)00423-4
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发表时间：
2000-10-15
期刊：
影响因子：
2.8
通讯作者：
Yoshimura, Y
中科院分区：
文献类型：
--
作者：
Isobe, N;Yoshimura, Y
我们检查了囊性、闭锁和健康的窦卵泡中增殖细胞的频率，以确定细胞增殖障碍是否与牛囊性卵泡的发生有关。用小鼠增殖细胞核抗原单克隆抗体（PCNA）对健康卵泡、闭锁卵泡和囊性卵泡的石蜡切片进行免疫染色。从卵泡顶端到卵泡底侧的4个不同区域均有pcna阳性细胞。在颗粒层中，健康卵泡基底区出现pcna阳性细胞的频率明显高于根尖区。在闭锁卵泡的颗粒层中观察到类似的pcna阳性细胞群模式，尽管闭锁卵泡基底区的频率明显低于健康卵泡。囊性卵泡颗粒层细胞的增殖速率在基底区明显低于闭锁卵泡。在卵泡内，早期闭锁卵泡中pcna阳性细胞的频率高于早期囊性卵泡。这些结果表明，在健康卵泡中，颗粒细胞的增殖活性在基底区高于根尖区，颗粒和内膜的细胞增殖活性可能随着卵泡囊肿的诱导而降低。(C) 2000年由爱思唯尔科学公司
We examined the frequency of proliferating cells in cystic, atretic and healthy antral follicles to determine whether a disorder of cell proliferation was responsible for the occurrence of bovine cystic follicles. Paraffin sections of healthy follicles and various stages of atretic and cystic follicles were immunostained with mouse monoclonal antibody to proliferating cell nuclear antigen (PCNA). The PCNA-positive cells were counted in 4 different regions of a follicle from the apical to the basal side. In the granulosa layer, a significantly higher frequency of PCNA-positive cells was observed in the healthy follicle in the basal region as compared with the apical region. A similar pattern of PCNA-positive cells population was observed in the granulosa layer of atretic follicles, although the frequency in the basal region was significantly lower in the atretic than the healthy follicle. The rate of cell proliferation in the granulosa layer of cystic follicles was markedly lower at the basal region than that of atretic follicles. In the theca interna, the frequency of PCNA-positive cells in atretic follicles at the early stages was higher than that in cystic follicles at the early stages. These results suggest that in the healthy follicle the proliferative activity of granulosa cells is higher in the basal than the apical region, and that the cell proliferation activity in the granulosa and theca interna may decrease in association with the induction of a follicular cyst. (C) 2000 by Elsevier Science Inc.