TRAF6 is upregulated in colon cancer and promotes proliferation of colon cancer cells
TRAF6 is upregulated in colon cancer and promotes proliferation of colon cancer cells
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TRAF6在结肠癌中表达上调并促进结肠癌细胞增殖
DOI：
10.1016/j.biocel.2014.04.010
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发表时间：
2014-08-01
影响因子：
4
通讯作者：
Fang, Jing
中科院分区：
文献类型：
--
作者：
Sun, Heng;Li, Xuebing;Fang, Jing
TNF受体相关因子6 （TRAF6）在先天免疫反应和适应性免疫反应的调控中起关键作用。尽管最近的研究表明TRAF6与癌症有关，但其在包括结肠癌在内的癌症中的确切作用尚未得到广泛研究，在很大程度上仍是未知的。本工作的目的是确定TRAF6在结肠癌中的表达及其在结肠癌细胞增殖和凋亡中的可能作用。对50个结肠癌组织与其邻近的非癌组织配对进行了分析。TRAF6在癌症中表达上调（P = 0.000），并与肿瘤分级相关（P = 0.012）。肿瘤组织阵列分析也表明，TRAF6在结肠癌中表达上调（P = 0.000），但TRAF6上调与患者生存率无相关性（P = 0.055）。我们发现TRAF6的下调通过细胞周期蛋白D1阻断结肠癌细胞的增殖。与其他报道不同的是，在我们的实验中，单独敲低TRAF6对结肠癌细胞的存活影响很小。TRAF6的下调使细胞对常规抗癌药物5-氟尿嘧啶和依托泊苷的治疗变得敏感。因此，抑制TRAF6可能会改善这些药物的治疗效果。总之，我们的数据表明TRAF6促进结肠癌细胞的增殖，它可能作为结肠癌治疗的潜在靶点。(C) 2014 Elsevier Ltd.版权所有。
TNF receptor associated factor 6 (TRAF6) plays a critical role in the regulation of innate immune responses and adaptive immune responses. Though recent studies indicate that TRAF6 is involved in cancer, its precise role in cancer including colon cancer has not been extensively investigated and remains largely unknown. The purpose of this work is to determine the expression of TRAF6 in colon cancer as well as the possible role of it in proliferation and apoptosis of colon cancer cells. Fifty colon cancer tissues paired with their adjacent non-cancerous tissues were analyzed. TRAF6 expression is upregulated in cancers (P = 0.000), which is correlated with tumor grades (P = 0.012). The tumor tissue-array analysis also indicates that expression of TRAF6 is upregulated in colon cancer (P = 0.000), but the TRAF6 upregulation has no association with patients' survival rate (P = 0.055). We found that knockdown of TRAF6 blocks proliferation of colon cancer cells through cyclin D1. Different from other reports, in our experiments knockdown of TRAF6 alone has little effect on survival of colon cancer cells examined. Knockdown of TRAF6 sensitizes the cells to treatment of the conventional anti-cancer drugs 5-fluorouracil and etoposide. Thus, inhibition of TRAF6 may improve the therapeutic treatment of these drugs. Together, our data suggest that TRAF6 promotes proliferation of colon cancer cells and it may serve as a potential target for therapy of colon cancer. (C) 2014 Elsevier Ltd. All rights reserved.