Inhibition of dentine caries using fluoride solution with silver nanoparticles: An in vitro study
Inhibition of dentine caries using fluoride solution with silver nanoparticles: An in vitro study
复制标题
使用含银纳米颗粒的氟化物溶液抑制牙本质龋齿：一项体外研究
DOI：
10.1016/j.jdent.2020.103512
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发表时间：
2020-12-01
影响因子：
4.4
通讯作者：
Chu, Chun Hung
中科院分区：
文献类型：
--
作者：
Yin, Iris Xiaoxue;Yu, Ollie Yiru;Chu, Chun Hung
**目的**：研究含银纳米颗粒（AgNPs）的氟化钠（NaF）对人工牙本质龋的再矿化及染色作用。
**方法**：将患有人工龋的人牙本质块分为四组。第1组使用含4000 ppm银纳米颗粒的5%氟化钠（22600 ppm氟化物）；第2组使用4000 ppm银纳米颗粒；第3组使用5%氟化钠；第4组使用去离子水（阴性对照）。所有组均经历三个生化循环。每个循环包括变形链球菌生物膜攻击和再矿化过程。使用显微计算机断层扫描、傅里叶变换红外光谱、扫描电子显微镜（SEM）和X射线衍射评估牙本质块的病损深度、矿物质 - 有机物含量、表面形态和晶体特征。使用分光光度法评估牙本质块的颜色变化。
**结果**：第1 - 4组的平均病损深度分别为151.13 ± 29.13μm、172.38 ± 23.44μm、190.41 ± 32.81μm和221.24 ± 27.91μm。第1 - 4组的磷酸氢根与酰胺I的比率分别为5.98 ± 0.36、3.86 ± 0.56、4.00 ± 0.67和2.53 ± 0.40。银纳米颗粒与氟化钠之间无显著交互作用。扫描电镜显示，与其他组相比，第1组牙本质胶原纤维暴露较少。X射线衍射显示第1组和第2组存在氯化银和金属银。四组之间的颜色变化无显著差异（p = 0.74）。
**结论**：含银纳米颗粒的氟化钠溶液可使牙本质龋再矿化且不产生染色。
**临床意义**：含银纳米颗粒的氟化钠溶液在龋齿治疗方面具有潜在应用价值。
Objectives: To investigate the remineralising and staining effects of sodium fluoride (NaF) with silver nanoparticles (AgNPs) on artificial dentine caries.Methods: Human dentine blocks with artificial caries were divided into four groups. Group 1 received 5 % NaF (22,600 ppm fluoride) with 4000 ppm AgNPs; group 2 received 4000 ppm AgNPs; group 3 received 5 % NaF, group 4 received deionised water (negative control). All groups underwent three biochemical cycles. Each cycle included Streptococcus mutans biofilm challenge and remineralisation process. The lesion depth, mineral-organic content, surface morphology and crystal characteristics of dentine blocks were evaluated using micro-computed tomography, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction. Colour change of dentine blocks was assessed using spectrophotometry.Results: The mean lesion depths of groups 1-4 were 151.13 +/- 29.13 mu m, 172.38 +/- 23.44 mu m, 190.41 +/- 32.81 mu m and 221.24 +/- 27.91 mu m, respectively. The hydrogen phosphate-to-amide I ratios of groups 1-4 were 5.98 +/- 0.36, 3.86 +/- 0.56, 4.00 +/- 0.67 and 2.53 +/- 0.40, respectively. There was no significant interaction effect between AgNPs and NaF. SEM showed less exposure of dentine collagen fibres in group 1 when compared to other groups. X-ray diffraction revealed presence of silver chloride and metallic silver in group 1 and 2. There was no significant difference in colour change among the four groups (p = 0.74).Conclusions: NaF solution with AgNPs can remineralise dentine caries without staining.Clinical Significance: Sodium fluoride solutions that include silver nanoparticles have potential uses in the management of caries.