Effect of pre-existing maternal obesity, gestational diabetes and adipokines on the expression of genes involved in lipid metabolism in adipose tissue
Effect of pre-existing maternal obesity, gestational diabetes and adipokines on the expression of genes involved in lipid metabolism in adipose tissue
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DOI：
10.1016/j.metabol.2013.10.001
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发表时间：
2014-02-01
影响因子：
9.8
通讯作者：
Lappas, Martha
中科院分区：
文献类型：
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作者：
Lappas, Martha
目标。探讨肥胖、妊娠期糖尿病（GDM）及脂肪因子对脂肪酸摄取、转运、合成及代谢相关基因表达的影响。研究人员从瘦、超重和肥胖的正常糖耐量（NGT）女性和GDM女性中获得了人体皮下和网膜脂肪组织。采用定量RT-PCR （qRT-PCR）检测表达水平。采用脂肪组织外植体测定脂己碱对TNF、il - 1的影响[3]。瘦素对脂肪组织基因表达的影响。先前存在的母体肥胖和GDM与脂肪酸摄取和细胞内运输（LPL、FATP2、FATP6、FABPpm和ASCL1）、甘油三酯（TAG）生物合成（MGAT1、7 MGAT2和DGAT1）、脂肪生成（FASN）和脂肪分解（PNPLA2、HSL和MGLL）相关基因的表达降低有关。参与脂质代谢的转录因子（LXR、PPAR、PPAR6、PPARy、RXR和SREBP1c）的基因表达也有所下降。另一方面，肥胖和GDM女性脂肪组织中脂肪因子TINTE、IL-113和瘦素的基因表达增加。体外功能研究表明，这些脂肪因子可降低LPL、FATP2、FATP6、ASCL1、PNPLA2、PPAR6、PPARy和rx11的基因表达。妊娠合并既往孕妇肥胖和GDM与脂肪组织脂质代谢异常相关，这可能在这些疾病的发病机制中发挥作用。(C) 2014爱思唯尔公司版权所有。
Objective. To determine the effect of maternal obesity, gestational diabetes mellitus (GDM) and adipokines on the expression of genes involved in fatty acid uptake, transport, synthesis and metabolism.Materials/Methods. Human subcutaneous and omental adipose tissues were obtained from lean, overweight and obese normal glucose tolerant (NGT) women and women with GDM. Quantitative RT-PCR (qRT-PCR) was performed to determine the level of expression. Adipose tissue explants were performed to determine the effect of the adipolcines TNF, IL1[3. and leptin on adipose tissue gene expression.Results. Pre-existing maternal obesity and GDM are associated with decreased expression in genes involved in fatty acid uptake and intracellular transport (LPL, FATP2, FATP6, FABPpm and ASCL1), triacylglyceride (TAG) biosynthesis (MGAT1,7 MGAT2 and DGAT1), lipogenesis (FASN) and lipolysis (PNPLA2, HSL and MGLL). Decreased gene expression was also observed for the transcription factors involved in lipid metabolism (LXR, PPAR, PPAR6, PPARy, RXR and SREBP1c). On the other hand, the gene expression of the adipokines TINTE, IL-113 and or leptin was increased in adipose tissue from obese and GDM women. Functional in vitro studies revealed that these adipokines decreased the gene expression of LPL, FATP2, FATP6, ASCL1, PNPLA2, PPAR6, PPARy and RX11.Conclusions. Pregnancies complicated by pre-existing maternal obesity and GDM are associated with abnormal adipose tissue lipid metabolism, which may play a role in the pathogenesis of these diseases. (C) 2014 Elsevier Inc. All rights reserved.