Vitamin E imaging and localization in the neuronal membrane
Vitamin E imaging and localization in the neuronal membrane
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DOI：
10.1021/ja051223y
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发表时间：
2005-09-07
影响因子：
15
通讯作者：
Sweedler, JV
中科院分区：
文献类型：
--
作者：
Monroe, EB;Jurchen, JC;Sweedler, JV
维生素E（α-生育酚）与几种细胞过程有关，包括信号传导、转运、脂质膜弯曲和几种神经退行性疾病。维生素E成像一直受到阻碍的分子难以进入传统的免疫组织化学方法。利用飞行时间二次离子质谱（ToF-SIMS），主要成分在分离的神经元细胞膜的分布进行了研究。与整个细胞相比，索马-神经突连接处的维生素E信号显著增加（在整个细胞中发现的165 ± 11%，p= 0.004，n = 12）。所观察到的膜分布表明维生素E在神经元功能中的重要新作用。
Vitamin E (α-tocopherol) has been implicated in several cellular processes including signaling, transport, lipid membrane curvature, and several neurodegenerative disorders. Vitamin E imaging has been hindered by the inaccessibility of the molecule to traditional immunohistochemical methods. Using time-of-flight secondary ion mass spectrometry (ToF-SIMS), the distribution of major constituents in the cellular membrane of isolated neurons was investigated. There is a significant increase in the vitamin E signal at the soma−neurite junction compared to the cell as a whole (165 ± 11% of that found across the cell,p= 0.004,n= 12). The observed membrane distribution suggests an important new role for vitamin E in neuronal function.