An immobilized biotin ligase:: Surface display of Escherichia coli BirA on Saccharomyces cerevisiae
An immobilized biotin ligase:: Surface display of Escherichia coli BirA on Saccharomyces cerevisiae
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DOI：
10.1021/bp050279t
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发表时间：
2005-11-01
影响因子：
2.9
通讯作者：
Boder, ET
中科院分区：
文献类型：
--
作者：
Parthasarathy, R;Bajaj, J;Boder, ET
The Escherichia coli biotin ligase enzyme BirA has been extensively used in recent years to generate site-specifically biotinylated proteins via a biotin acceptor peptide tag. In the present study, BirA was displayed for the first time on the yeast Saccharomyces cerevisiae using the Aga1p-Aga2p platform and assayed using a peptide-tagged protein as the substrate. The enzyme is fully functional and resembles the soluble form in many of its properties, but the yeast-displayed enzyme demonstrates stability and reusability on the time scale of weeks. Thus, the yeast-displayed BirA system represents a facile and highly economical alternative for producing site-specifically biotinylated proteins.
