Spectral characteristics of the PhNR in the full-field flash electroretinogram of normals and glaucoma patients
Spectral characteristics of the PhNR in the full-field flash electroretinogram of normals and glaucoma patients
复制标题
DOI：
10.1007/s10633-011-9304-z
复制
发表时间：
2012-04-01
影响因子：
1.4
通讯作者：
Horn, Folkert K.
中科院分区：
文献类型：
--
作者：
Kremers, Jan;Jertila, Mounira;Horn, Folkert K.
对正常受试者在不同颜色的闪光和背景组合下的闪光视网膜电图反应进行了测量。在不同的闪光强度下测量了闪光响应分量的振幅，并可以用广义的Naka-Rushton函数来描述。在不同背景亮度下重复测量以研究适应效应。研究发现，当闪光强度和背景亮度分别以光度法表示(cd s/m（2)和cd/m(2)）时，除了蓝色（峰值波长458 nm）在橙色（峰值波长591 nm）背景上闪烁外，所有颜色组合的响应都非常相似。我们提出，在这种情况下，第二个（可能是s锥或杆驱动）机构侵入。b波后的负反应（这里称为“光负反应”或PhNR）被认为反映了神经节细胞的活性，并且在这种情况下也是最大的。在39名正常受试者和49名青光眼患者中测量了在591 nm背景下458nm闪光和相反组合的反应。青光眼对PhNR波幅均有影响。反映反应和适应动态的其他组成参数没有改变。在分离正常人和患者PhNR振幅的条件中，最好的刺激条件是在10 cd/m(2) 591 nm背景下1 cd s/m(2) 458nm的闪光。
Flash electroretinogram responses were measured in normal subjects to different chromatic combinations of flashes and backgrounds. The amplitudes of the flash response components were measured at different flash strengths and could be described by a generalized Naka-Rushton function. The measurements were repeated at different background luminances to study adaptation effects. It was found that when flash strength and background luminance were expressed in photometric terms (cd s/m(2) and cd/m(2), respectively), then the responses were very similar for all chromatic combinations with the exception of the condition in which blue (peak wavelength 458 nm) was flashed upon an orange (peak wavelength 591 nm) background. We propose that in this condition, a second (possibly S-cone or rod-driven) mechanism intrudes. The negative response after the b-wave (here called "photopic negative response" or PhNR for all conditions) is thought to reflect ganglion cell activity and was also largest at this condition. Responses were measured to the 458 nm flash on 591 nm background and the reversed combination in a population of 39 normal subjects and 49 glaucoma patients. It was found that the PhNR amplitude was affected by glaucoma in all conditions. Other component parameters, reflecting responses and adaptation dynamics, were not altered. The best stimulus condition among the conditions used to separate the PhNR amplitude of normals and patients was a 1 cd s/m(2) 458 nm flash on a 10 cd/m(2) 591 nm background.