Danger peptide signaling enhances internalization of a geminivirus symptom determinant in plant cells during infection
Danger peptide signaling enhances internalization of a geminivirus symptom determinant in plant cells during infection
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危险肽信号传导增强感染期间植物细胞中双病毒症状决定因素的内化
DOI：
10.1093/jxb/eraa053
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发表时间：
2020
影响因子：
6.9
通讯作者：
Lai Jianbin
中科院分区：
文献类型：
--
作者：
Zeng Runxiu;Liu Xiaoshi;Li Huiyun;Wu Shuang;Huang Wei;Zhai Zhenqian;Li Min;Lin Yuxin;Xie Qi;Yang Chengwei;Lai Jianbin
双生病毒是DNA病毒，其在不同种类的植物中引起严重疾病，导致全世界相当大的农业损失。C4蛋白是甜菜严重曲顶病毒（BSCTV）等双生病毒的主要症状决定因子。在这里，我们发现了一种新的机制，通过这种机制，危险肽信号增强了BSCTV C4在植物细胞中的内化。以前的研究表明，这种信号对于激活细菌和真菌触发的免疫反应很重要，但它在植物-病毒相互作用中的功能以前是未知的。Pep 1受体1（PEPR 1）和PEPR 2是在危险肽途径中由Peps（植物激发肽）识别的受体激酶。我们发现BSCTV C4上调PEPR 2，但不与PEPR 1相互作用。Pep 1-PEPR 2复合物在拟南芥和烟草本氏细胞中都能刺激C4的内化。此外，C4诱导拟南芥愈伤组织的形成，这是由PEPR 2过表达抑制，但在pepr 2突变体增强。在Pep 1存在的情况下，PEPR 2的过表达抑制了N.本萨米亚纳外源Pep 1还以aPEPR 2依赖的方式降低拟南芥中BSCTV的感染。因此，PEPR 2识别症状决定因子C4并增强其由危险肽介导的内化，从而抑制BSCTV感染。
Geminiviruses are DNA viruses that cause severe diseases in diverse species of plants, resulting in considerable agricultural losses worldwide. C4 proteins are a major symptom determinant in several geminiviruses, includingBeet severe curly top virus(BSCTV). Here, we uncovered a novel mechanism by which danger peptide signaling enhances the internalization of BSCTV C4 in plant cells. Previous studies showed that this signaling is important for activation of bacterium- and fungus-triggered immune responses, but its function in plant–virus interactions was previously unknown. Pep1 RECEPTOR1 (PEPR1) and PEPR2 are receptor kinases recognized by Peps (plant elicitor peptides) in the danger peptide pathway. We found that BSCTV C4 up-regulated and interacted with PEPR2 but not PEPR1. The Pep1–PEPR2 complex stimulated the internalization of C4 in both Arabidopsis andNicotiana benthamianacells. Furthermore, C4 induced callus formation in Arabidopsis, which was suppressed byPEPR2overexpression but enhanced in thepepr2mutants. In the presence of Pep1, overexpression ofPEPR2suppressed BSCTV infection inN. benthamiana. Exogenous Pep1 also reduced BSCTV infection in Arabidopsis in aPEPR2-dependent manner. Thus, PEPR2 recognizes the symptom determinant C4 and enhances its internalization mediated by danger peptides, suppressing BSCTV infection.