The polycystic kidney disease 1 (Pkd1) gene is required for the responses of osteochondroprogenitor cells to midpalatal suture expansion in mice
The polycystic kidney disease 1 (Pkd1) gene is required for the responses of osteochondroprogenitor cells to midpalatal suture expansion in mice
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DOI：
10.1016/j.bone.2009.02.018
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发表时间：
2009-06-01
期刊：
影响因子：
4.1
通讯作者：
Olsen, Bjorn R.
中科院分区：
文献类型：
--
作者：
Hou, Bo;Kolpakova-Hart, Elona;Olsen, Bjorn R.
已知机械应力调节出生后骨骼的生长和发育。然而，机械转导的机制尚未完全了解。多囊蛋白-1（PC 1）是可能在该过程中发挥作用的蛋白质中有前途的候选者，因为它已被证明在肾上皮中起流量传感器的作用，并且已知其对骨骼发育很重要。为了研究PC 1是否参与骨骼组织的机械传导，对神经嵴细胞、成骨细胞或软骨细胞中PC 1条件性缺陷的小鼠进行了腭中缝扩张。动态骨标记显示，在Wnt 1Cre; Pkd 1小鼠中，对扩张反应的新骨形成显著减少，因为在2周时，突变小鼠中含有新骨的缝合面积为14.0 +/- 3.4%，而对照小鼠为65.0 +/- 3.8%（p
Mechanical stress is known to modulate postnatal skeletal growth and development. However, the mechanisms underlying the mechanotransduction are not fully understood. Polycystin-1 (PC1) is a promising candidate among proteins that may play a role in the process as it has been shown to function as a flow sensor in renal epithelium and it is known to be important for skeletal development. To investigate whether PC1 is involved in mechanotransduction in skeletal tissues, mice with a conditional deficiency for PC1 in neural crest cells, osteoblasts or chondrocytes were subjected to midpalatal suture expansion. Dynamic bone labeling revealed that new bone formation in response to expansion was significantly reduced in Wnt1Cre;Pkd1 mice, as the suture area containing new bone was 14.0 +/- 3.4% in mutant mice versus 65.0 +/- 3.8% in control mice at 2 weeks (p