Distribution of oncofetal antigen tumor-associated glycoprotein-72 defined by monoclonal antibody B72.3.
Distribution of oncofetal antigen tumor-associated glycoprotein-72 defined by monoclonal antibody B72.3.
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发表时间：
1986-06
期刊：
影响因子：
11.2
通讯作者：
A. Thor;N. Ohuchi;C. Szpak;W. Johnston;J. Schlom
中科院分区：
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作者：
A. Thor;N. Ohuchi;C. Szpak;W. Johnston;J. Schlom
使用抗生物素蛋白-生物素复合物免疫组织化学技术，针对人转移癌的膜富集提取物制备鼠单克隆抗体 B72.3，与一系列成人和胎儿人体组织反应，以评估反应性肿瘤相关糖蛋白 (TAG)-72 抗原的表达。 TAG-72 在多种上皮源性癌症中表达，包括 94% 的结肠腺癌、84% 的乳腺癌浸润性导管癌、96% 的非小细胞肺癌、100% 的常见上皮性卵巢癌以及大多数胰腺癌、胃癌和食道癌 评价。然而，在神经、造血或肉瘤来源的肿瘤中未观察到TAG-72表达，这表明TAG-72抗原本质上是“全癌”。在成人正常组织中通常未观察到明显的单克隆抗体 B72.3 反应性，在乳腺和结肠的一些良性病变中观察到有限的反应性。然而，在胎儿结肠、胃和食道中检测到 TAG-72 抗原表达，因此将 TAG-72 定义为癌胎儿抗原。 TAG-72 先前已被证明与癌胚抗原和其他肿瘤相关抗原不同。单克隆抗体 B72.3 的全癌分布以及与正常成人组织缺乏显着反应性表明其对人类癌症具有潜在的诊断和治疗用途。
Murine monoclonal antibody B72.3, prepared against a membrane-enriched extract of human metastatic carcinoma, was reacted with a spectrum of adult and fetal human tissues using avidin-biotin-complex immunohistochemical techniques to evaluate the expression of the reactive tumor associated glycoprotein (TAG)-72 antigen. TAG-72 was shown to be expressed in several epithelial-derived cancers including 94% of colonic adenocarcinomas, 84% of invasive ductal carcinomas of the breast, 96% of non-small cell lung carcinomas, 100% of common epithelial ovarian carcinomas, as well as the majority of pancreatic, gastric, and esophageal cancers evaluated. TAG-72 expression was not observed, however, in tumors of neural, hematopoietic, or sarcomatous derivation, suggesting that the TAG-72 antigen is "pancarcinoma" in nature. Appreciable monoclonal antibody B72.3 reactivity was generally not observed in adult normal tissues, with limited reactivity noted in a few benign lesions of the breast and colon. TAG-72 antigen expression was detected, however, in fetal colon, stomach, and esophagus, thus defining TAG-72 as an oncofetal antigen. TAG-72 has previously been shown to be distinct from carcinoembryonic antigen and other tumor associated antigens. The pancarcinoma distribution and lack of significant reactivity with normal adult tissues of monoclonal antibody B72.3 suggest its potential diagnostic and therapeutic utility for human carcinomas.