Age-related changes in mouse bone permeability
Age-related changes in mouse bone permeability
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小鼠骨通透性与年龄相关的变化
DOI：
10.1016/j.jbiomech.2013.12.020
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发表时间：
2014
影响因子：
2.4
通讯作者：
Rodriguez-Florez N
中科院分区：
文献类型：
--
作者：
Rodriguez-Florez N
腔隙-小管渗透性的测定对于理解骨中的局部流体流动是必不可少的，这可以指示骨感觉如何在机械环境中变化以调节机械适应。在文献中发现的腔隙-小管渗透性的估计值变化高达8个数量级，并且在非骨细胞小鼠骨中尚未测量到与年龄相关的渗透性变化。本研究的目的是使用基于纳米压痕数据的多孔弹性方法来表征小鼠骨中腔隙-小管渗透性随年龄的变化。使用9只不同年龄（2、7和12个月）的野生型C57 BL/6小鼠。将每个年龄组的3个胫骨标本包埋在环氧树脂中，切成两半，并使用两个球形流体压头（R=238 μm和500 μm）在皮质中部纵向压痕。结果表明，小鼠骨的腔隙-小管固有渗透性在2 ~ 7个月时降低，7 ~ 12个月时无明显变化。大压头尖端施加更大的接触尺寸和采样更大范围的渗透率，特别是对于旧骨。对于具有较小半径尖端的压痕，未观察到这种与年龄相关的分布差异。我们得出结论，小尖端有效地测量腔隙-小管渗透性，而较大的尖端缩进血管渗透性的影响。探索与年龄相关的变化，骨渗透性测量纳米压痕将导致更好地理解的作用，流体流动的力学转导。这种理解可能有助于指示骨适应和重塑的改变。
The determination of lacunar-canalicular permeability is essential for understanding local fluid flow in bone, which may indicate how bone senses changes in the mechanical environment to regulate mechano-adaptation. The estimates of lacunar-canalicular permeability found in the literature vary by up to eight orders of magnitude, and age-related permeability changes have not been measured in non-osteonal mouse bone. The objective of this study is to use a poroelastic approach based on nanoindentation data to characterize lacunar-canalicular permeability in murine bone as a function of age. Nine wild type C57BL/6 mice of different ages (2, 7 and 12 months) were used. Three tibiae from each age group were embedded in epoxy resin, cut in half and indented in the longitudinal direction in the mid-cortex using two spherical fluid indenter tips (R=238 μm and 500 μm). Results suggest that the lacunar-canalicular intrinsic permeability of mouse bone decreases from 2 to 7 months, with no significant changes from 7 to 12 months. The large indenter tip imposed larger contact sizes and sampled larger ranges of permeabilities, particularly for the old bone. This age-related difference in the distribution was not seen for indents with the smaller radius tip. We conclude that the small tip effectively measured lacunar-canalicular permeability, while larger tip indents were influenced by vascular permeability. Exploring the age-related changes in permeability of bone measured by nanoindentation will lead to a better understanding of the role of fluid flow in mechano-transduction. This understanding may help indicate alterations in bone adaptation and remodeling.