The cultivation of vaccine and other viruses in the chorio-allantoic membrane of chick embryos
The cultivation of vaccine and other viruses in the chorio-allantoic membrane of chick embryos
复制标题
DOI：
10.1126/science.74.1919.371
复制
发表时间：
1931-10-09
期刊：
影响因子：
56.9
通讯作者：
Buddingh, G. J.
中科院分区：
文献类型：
--
作者：
Goodpasture, E. W.;Woodruff, A. M.;Buddingh, G. J.
从受感染的膜上取下的涂片，经常可以看到细胞质内有一团部分分散的帕邢小体;在蒸馏水中检查的膜碎片，可以看到圆形或椭圆形的团块，在团块中可以看到快速布朗运动的微小小体，就像在鸡痘和锥状软疣的内含物中看到的一样。目前正在研究帕邢体与瓜涅里体结构的关系。这两种元素的丰富和后者的不寻常的大尺寸使这种组织特别适合这样的调查。将病毒在鸡胚中传八代，在拔毛后涂于2 ~ 3日龄雏鸡的皮肤上。接种部位3天内出现直径约1 mm的肉眼可见结节。在两三天的时间里，它们显然是静止不动的，它们迅速后退。从牛痘中恢复的小鸡在两周后感染鸡痘病毒，病变的发展与正常小鸡的鸡痘完全一样。雏鸡体内的瓦氏杆菌对鸡痘不产生免疫力。
Smears made from infected membrane frequently show masses of partially dispersed Paschen bodies within the cytoplasm of cells; and pieces of mem-brane examined in distilled water show round or oval masses in which minute bodies are to be seen in rapid Brownian motion, just like those seen in the inclusions of fowl-pox and Molluscum con, tagiosum. Studies are being made upon the relation of the Paschen bodies to the structure of Guarnieri bodies. The abundance of both these elements and the un-usually large size of the latter make this tissue especially adaptable to such an investigation. Virus passed through eight generations in chick embryos has been appliedto the skin of two or three day old chicks after plucking the down. Macroscopic nodules about 1 mm in diameter appear at the site of inoculation within three days. Remaining apparently stationary for two or three days they rapidly recede. Chicks recovered from vaccinia were infected two weeks later with fowl-pox virus and the lesions evoluted exactly like those of fowl-pox in normal chicks. Vaceinia in the chick induces no immunity to fowl-pox.