Complementary and antagonistic effects of IL-3 in the early development of human megakaryocytes in culture.
Complementary and antagonistic effects of IL-3 in the early development of human megakaryocytes in culture.
复制标题
IL-3 在培养的人类巨核细胞早期发育中的互补和拮抗作用。
DOI：
10.1046/j.1365-2141.1998.00579.x
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发表时间：
1998
影响因子：
6.5
通讯作者：
Karpatkin,S
中科院分区：
文献类型：
--
作者：
Dolzhanskiy,A;Hirst,J;Basch,RS;Karpatkin,S
本文研究了IL-3在巨核细胞(MK)生长发育早期的作用。尽管血小板生成素(TPO)本身可以支持成熟的CD41+MK由前MK向MK方向发展，但IL-3和SCF的加入可显著增加CD41+MK的生成。IL-3也能支持半固体培养基集落生长(CFU-MK)。在含有IL-3的悬浮培养中培养的CD41+细胞与在没有这种试剂的情况下培养的CD41+细胞的表型不同。与不加IL-3相比，在IL-3作用下生长的细胞更快地丧失CD34的表达，表达较低水平的血小板膜糖蛋白GPIIb-IIIa和Ib，并获得较低程度的多倍体。IL-3的抑制作用不是由于在IL-3不刺激MK生长的条件下观察到的未成熟细胞数量的增加而稀释成熟细胞所致。这些培养的结果表明，IL-3在早期MK发育中起重要作用，但在内复制开始后抑制进一步成熟。因此，长时间接触IL-3会导致出现不能正常成熟的细胞。
The effect of IL‐3 on the early steps in the growth and development of megakaryocytes (MK) in culture has been studied. Although thrombopoietin (TPO) by itself could support the development of mature CD41+MK from pre‐MK, the number of cells produced was greatly augmented by the addition of IL‐3 and SCF. IL‐3 was also able to support the growth of MK colonies in semi‐solid media (CFU‐MK). The CD41+cells that developed in suspension cultures containing IL‐3 differed phenotypically from those that developed without this agent. Cells grown in the presence of IL‐3 lost CD34 expression more rapidly, expressed lower levels of the platelet glycoproteins gpIIb‐IIIa and Ib and achieved lower degrees of polyploidy than in the absence of IL‐3. The inhibitory effects of IL‐3 were not a consequence of the dilution of the mature cells by increased numbers of immature cells since it was observed under conditions in which IL‐3 did not stimulate MK growth. The results obtained in these cultures suggest that IL‐3 plays an important role in early MK development, but inhibits further maturation after endoreduplication begins. Thus, prolonged contact with IL‐3 results in the appearance of cells that do not mature normally.