The genome phylogeny of domestic cat, red panda and five mustelid species revealed by comparative chromosome painting and G-banding
The genome phylogeny of domestic cat, red panda and five mustelid species revealed by comparative chromosome painting and G-banding
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DOI：
10.1023/a:1015292005631
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发表时间：
2002-03-01
影响因子：
2.6
通讯作者：
Yang, FT
中科院分区：
文献类型：
--
作者：
Nie, WH;Wang, JH;Yang, FT
石貂全基因组同源图谱的构建（Martes foina，2n = 38），家猫（Felis卡图斯，2n = 38），美国水貂（Mustela vison，2n = 30），黄喉貂（Martes flavigula，2n = 40），旧大陆獾用一套完整的石貂探针进行种间染色体涂染，建立了黑貂（Meles meles，2n = 44）、雪貂（Melogale moschata，2n = 38）和小熊猫（Ailurus fulgens，2n = 36）的染色体组型。18个石貂常染色体探针分别在美国貂、黄喉貂、美洲獾、雪貂和小熊猫的基因组中发现了20、19、21、18和21对同源染色体片段。石貂和猫之间的相互绘画描绘了石貂和猫基因组中共有的21对同源片段。染色体彩绘结果表明，这些物种的大多数染色体是高度保守的，并显示与石貂和猫染色体或染色体臂一一对应，只有少数染色体间重排（罗伯逊融合和分裂）发生在物种辐射。通过比较这些物种和假定的祖先食肉动物核型中保守染色体片段的分布模式，我们重建了这些物种从假定的2n = 42祖先食肉动物核型进化的途径。我们的研究结果支持小熊猫和鼬科动物之间的亲缘关系。这些图谱中的同源性数据将使我们能够将猫基因定位数据转移到其他未定位的食肉动物物种。
Genome-wide homology maps among stone marten (Martes foina, 2n = 38), domestic cat (Felis catus, 2n = 38), American mink (Mustela vison, 2n = 30), yellow-throated marten (Martes flavigula, 2n = 40), Old World badger (Meles meles, 2n = 44), ferret badger (Melogale moschata, 2n = 38) and red panda (Ailurus fulgens, 2n = 36) have been established by cross-species chromosome painting with a complete set of stone marten probes. In total, 18 stone marten autosomal probes reveal 20, 19, 21, 18 and 21 pairs of homologous chromosomal segments in the respective genomes of American mink, yellow-throated marten, Old World badger, ferret badger and red panda. Reciprocal painting between stone marten and cat delineated 21 pairs of homologous segments shared in both stone marten and cat genomes. The chromosomal painting results indicate that most chromosomes of these species are highly conserved and show one-to-one correspondence with stone marten and cat chromosomes or chromosomal arms, and that only a few interchromosomal rearrangements (Robertsonian fusions and fissions) have occurred during species radiation. By comparing the distribution patterns of conserved chromosomal segments in both these species and the putative ancestral carnivore karyotype, we have reconstructed the pathway of karyotype evolution of these species from the putative 2n = 42 ancestral carnivore karyotype. Our results support a close phylogenetic relationship between the red panda and mustelids. The homology data presented in these maps will allow us to transfer the cat gene mapping data to other unmapped carnivore species.