COLLISION-INDUCED DISSOCIATION IN A TANDEM TIME-OF-FLIGHT MASS-SPECTROMETER WITH 2 SINGLE-STAGE REFLECTRONS
COLLISION-INDUCED DISSOCIATION IN A TANDEM TIME-OF-FLIGHT MASS-SPECTROMETER WITH 2 SINGLE-STAGE REFLECTRONS
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DOI：
10.1002/oms.1210281025
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发表时间：
1993-10-01
期刊：
影响因子：
--
通讯作者：
COTTER, RJ
中科院分区：
文献类型：
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作者：
CORNISH, TJ;COTTER, RJ
研制了一台串联飞行时间质谱仪，用于研究多肽和小分子蛋白质的碰撞诱导解离（CID）产物。该仪器包括两个单级反射器，第一个用于能量聚焦待碎裂的前体离子，第二个用于能量聚焦和分散碰撞池中形成的产物离子。使用单级而不是两级反射器，因为总透射率更高，与栅线场的干扰最小化，并且产物离子光谱的质量校准与时间完全线性。获得了罗丹明和几种肽的CID产物离子谱，发现其碎片模式与磁扇形质谱仪的B/E连接扫描模式中获得的碎片模式非常相似。
A tandem time-of-flight mass spectrometer was developed to be used for the study of collision-induced dissociation (CID) products of peptides and small proteins. The instrument incorporates two single-stage reflectrons, the first to energy focus the precursor ions to be fragmented and the second to both energy focus and disperse the product ions formed in the collision cell. Single-stage rather than two stage reflectrons were used because the overall transmission is higher, interferences with grid wire fields are minimized and the mass calibration of product ion spectra is exactly linear with time. The CID product ion spectra of rhodamine and several peptides were acquired and found to exhibit very similar fragmentation patterns to those acquired in the B/E linked scan mode of a magnetic sector mass spectrometer.