Adaptive divergence in experimental populations of Pseudomonas fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness.
Adaptive divergence in experimental populations of Pseudomonas fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness.
复制标题
荧光假单胞菌实验种群的适应性分歧。
作者：
A. Spiers;Sophie G Kahn;J. Bohannon;M. Travisano;P. Rainey
所有适应性辐射的一个中心特征是形态差异，但相关的表型创新鲜为人知。当在空间结构的环境中选择时，荧光假单胞菌的种群迅速分化。在这些不同的变种中，有一种突变型被称为“皱纹扩散器”(WS)，它通过形成自我支持的生物膜来定居一个新的生态位。通过筛选小生境缺陷(WS(-))突变体的WS转座子文库，寻找与初级表型创新有关的基因座。对一组突变体的详细分析揭示了一个由10个基因组成的操纵子，这些基因编码产生纤维素样聚合物(CLP)所需的酶。WS基因型过量产生CLP，聚合物的过度生产是WS菌落独特形态所必需的；它也是生物膜形成和空间结构微观世界中最大适合度所必需的，但过度生产CLP本身并不足以引起WS。一个工作模型预测，改变CLP产生的细胞周期控制是表型创新的重要决定因素。对表达WS表型所需的30kb DNA编码特性的分析，包括一个调节基因座，没有揭示突变原因，这表明一个复杂的基因-表型图谱。
A central feature of all adaptive radiations is morphological divergence, but the phenotypic innovations that are responsible are rarely known. When selected in a spatially structured environment, populations of the bacterium Pseudomonas fluorescens rapidly diverge. Among the divergent morphs is a mutant type termed "wrinkly spreader" (WS) that colonizes a new niche through the formation of self-supporting biofilms. Loci contributing to the primary phenotypic innovation were sought by screening a WS transposon library for niche-defective (WS(-)) mutants. Detailed analysis of one group of mutants revealed an operon of 10 genes encoding enzymes necessary to produce a cellulose-like polymer (CLP). WS genotypes overproduce CLP and overproduction of the polymer is necessary for the distinctive morphology of WS colonies; it is also required for biofilm formation and to maximize fitness in spatially structured microcosms, but overproduction of CLP alone is not sufficient to cause WS. A working model predicts that modification of cell cycle control of CLP production is an important determinant of the phenotypic innovation. Analysis of >30 kb of DNA encoding traits required for expression of the WS phenotype, including a regulatory locus, has not revealed the mutational causes, indicating a complex genotype-phenotype map.
DOI：
10.1073/pnas.040565697
发表时间：
2000-02-29
影响因子：
11.1
作者：
Blanton, RL;Fuller, D;Loomis, WF
通讯作者：
Loomis, WF
利福平耐药大肠杆菌的补偿性进化。
影响因子：
3.3
作者：
Reynolds,MG
通讯作者：
Reynolds,MG
编码大肠杆菌酰基载体蛋白的基因位于脂肪酸生物合成基因簇内。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rawlings,M;CronanJr,JE
通讯作者：
CronanJr,JE