The Hepatitis Delta Virus accumulation requires paraspeckle components and affects NEAT1 level and PSP1 localization.
The Hepatitis Delta Virus accumulation requires paraspeckle components and affects NEAT1 level and PSP1 localization.
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DOI：
10.1038/s41598-018-24500-1
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发表时间：
2018-04-16
影响因子：
4.6
通讯作者：
Pelchat M
中科院分区：
文献类型：
--
作者：
Beeharry Y;Goodrum G;Imperiale CJ;Pelchat M
The Hepatitis Delta Virus (HDV) relies mainly on host proteins for its replication. We previously identified that PSF and p54nrb associate with the HDV RNA genome during viral replication. Together with PSP1, these proteins are part of paraspeckles, which are subnuclear bodies nucleated by the long non-coding RNA NEAT1. In this work, we established the requirement for PSF, p54nrb and PSP1 in HDV replication using RNAi-mediated knockdown in HEK-293 cells replicating the HDV RNA genome. We determined that HDV replication induces the delocalization of PSP1 to cytoplasmic foci containing PABP and increases NEAT1 level causing an enlargement of NEAT1 foci. Overall, our data support a role for the main paraspeckles proteins in HDV life cycle and indicate that HDV replication causes a cellular stress and induces both a delocalization of the PSP1 to the cytoplasm and a disruption of paraspeckles.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.3390/v2010189
发表时间：
2010-01
期刊：
Viruses
影响因子：
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通讯作者：
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作者：
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