EVOLUTION OF THE VACUOLAR H+-ATPASE - IMPLICATIONS FOR THE ORIGIN OF EUKARYOTES
EVOLUTION OF THE VACUOLAR H+-ATPASE - IMPLICATIONS FOR THE ORIGIN OF EUKARYOTES
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DOI：
10.1073/pnas.86.17.6661
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发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
YOSHIDA, M
中科院分区：
文献类型：
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作者：
GOGARTEN, JP;KIBAK, H;YOSHIDA, M
真核细胞内膜系统的液泡膜组分之间的主动运输是由一种特殊的液泡膜H ~+-ATP酶激活的。液泡H+-ATP酶的70-和60-kDa亚基的氨基酸序列如下：25%相同的β。和α。亚基，分别为真细菌型F0 F1-ATP酶。我们现在报告，相同的液泡H+-ATP酶亚基。50%相同的α。和β亚基，分别是硫代谢酸热硫化叶菌，一种蛛形纲细菌（古细菌）。此外，在70-kDa亚基的氨基末端附近的88-氨基酸段上的同源物不存在于F0 F1-ATP酶β中。亚基，但存在于α亚基中。Sulfolobus亚单位。由于两种类型的亚基（α.和β亚单位; 60-和70-kDa亚基）是彼此同源的，它们一定是在真细菌、真核生物和硫化叶菌的最后共同祖先之前发生的基因复制引起的。因此，亚基的系统发育树可以扎根于基因复制发生的位点。推断的进化树包含两个主要分支：真细菌分支和产生硫化叶菌和真核宿主细胞的卵细胞分支。这意味着真核生物的液泡H+-ATP酶是由原始细胞质膜H+-ATP酶的内化而产生的。
Active transport across the vacuolar components of teh eukaryotic endomembrane system is energized by a specific vacuolar H+-ATPase. The amino acid sequences of the 70- and 60-kDa subunits of the vacuolar H+-ATPase are .apprxeq. 25% identical to the .beta. and .alpha. subunits, respectively, of the eubacterial-type F0F1-ATPases. We now report that the same vacuolar H+-ATPase subunits are .apprxeq. 50% identical to the .alpha. and .beta. subunits, respectively, of the sulfur-metabolizing Sulfolobus acidocaldarius, an arachabacterium (Archaeobacterium). Moreover, the homologue on an 88-amino acid stretch near the amino-terminal end of the 70-kDa subunit is absent from the F0F1-ATPase .beta. subunit but is present in the .alpha. subunit of Sulfolobus. Since the two types of subunits (.alpha. and .beta. subunits; 60- and 70-kDa subunits) are homologous to each other, they must have arisen by a gene duplication that occurred prior to the last common ancestor of the eubacteria, eukaryotes, and Sulfolobus. Thus, the phylogenetic tree of the subunits can be rooted at the site where the gene duplication occurred. The inferred evolutionary tree contains two main branches: a eubacterial branch and an eocyte branch that gave rise to Sulfolobus and the eukaryotic host cell. The implication is that the vacuolar H+-ATPase of eukaryotes arose by the internalization of the plasma membrane H+-ATPase of an archabacterial-like ancestral cell.