Protein structures forming the shell of primitive bacterial organelles
Protein structures forming the shell of primitive bacterial organelles
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DOI：
10.1126/science.1113397
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发表时间：
2005-08-05
期刊：
影响因子：
56.9
通讯作者：
Yeates, TO
中科院分区：
文献类型：
--
作者：
Kerfeld, CA;Sawaya, MR;Yeates, TO
细菌微区室是完全由蛋白质亚单位组成的原始细胞器。基因组序列数据库揭示了微区室在不同微生物中的广泛存在。原型细菌微区室是羧基体，一种用于螯合碳固定反应的蛋白质外壳。我们报告的三维晶体结构的多个羧基壳蛋白，揭示了一个六聚体单元作为基本的微区室构建块，并显示这些六聚体组装形成平面的多面体壳。这些结构表明了分子如何通过外壳运输，以及结构变化如何控制这些亚细胞区室的组装和结构。
Bacterial microcompartments are primitive organelles composed entirely of protein subunits. Genomic sequence databases reveal the widespread occurrence of microcompartments across diverse microbes. The prototypical bacterial microcompartment is the carboxysome, a protein shell for sequestering carbon fixation reactions. We report three-dimensional crystal structures of multiple carboxysome shell proteins, revealing a hexameric unit as the basic microcompartment building block and showing how these hexamers assemble to form flat facets of the polyhedral shell. The structures suggest how molecular transport across the shell may be controlled and how structural variations might govern the assembly and architecture of these subcellular compartments.