Heteronuclear two-dimensional 15N- and 13C-NMR studies of DNA oligomers and their netropsin complexes using indirect proton detection.
Heteronuclear two-dimensional 15N- and 13C-NMR studies of DNA oligomers and their netropsin complexes using indirect proton detection.
复制标题
使用间接质子检测对 DNA 寡聚物及其 netropsin 复合物进行异核二维 15N 和 13C-NMR 研究。
DOI：
10.1002/bip.360310105
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Cowburn,D
中科院分区：
文献类型：
--
作者：
Ashcroft,J;Live,DH;Patel,DJ;Cowburn,D
Heteronuclear multispin coherence proton‐detected two‐dimensional nmr spectroscopic experiments were used to obtain information on protonated carbons and nitrogens of the self‐complementary d (G‐G‐T‐A‐T‐A‐C‐C) and d (G‐G‐A‐A‐T‐T‐C‐C) duplexes, with and without the drug netropsin dissolved in aqueous solution. Many correlations of protons coupled to13C nuclei on the base and sugar rings of the octanucleotides were detected, allowing the carbon resonances to be assigned based on previous homonuclear proton two‐dimensional nmr studies. Imino nitrogen assignments can also be made using the proton assignments from previous one‐dimensional nuclear overhauser effect experiments. Imino nitrogen shifts may be useful indicators of changes in local hydrogen‐bonding interactions to base‐pair edges.
