Indices of lipid peroxidation in vivo: Strengths and limitations
Indices of lipid peroxidation in vivo: Strengths and limitations
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DOI：
10.1016/s0891-5849(00)00232-x
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发表时间：
2000-06-15
影响因子：
7.4
通讯作者：
Fitzgerald, GA
中科院分区：
文献类型：
--
作者：
Meagher, EA;Fitzgerald, GA
氧化应激被广泛认为是疾病的一种机制，但抗氧化剂的临床试验并没有包括剂量选择或患者入选的生化基础。传统上用于评估脂质过氧化的许多指标依赖于与体内事件相关性可疑的离体系统中进行的测量。常用的体内脂质过氧化指标受到目标anylate的非特异性或不稳定性、离体事件对anylate的污染以及分析方法的非特异性等问题的限制。最近，基于质谱的特定方法已应用于人体生物体液中的4-羟基壬烯醛和各种异前列烷。尿液中这些化合物的测量反映了体内的脂质过氧化反应，并提供了一种无创的方法，可以很容易地应用于临床试验。(C)2000 Elsevier Science Inc.
Oxidant stress has been widely implicated as a mechanism of disease, yet clinical trials of antioxidants have not included a biochemical basis for dose selection or patient inclusion. Many of the indices traditionally employed to assess lipid peroxidation have relied on measurements performed in ex vivo systems of questionable relevance to events in vivo. Commonly employed in vivo indices of lipid peroxidation are constrained by such issues as the nonspecificity or instability of the target anylate, contamination of the anylate by events ex vivo, and nonspecificity of analytical methodology. More recently, specific methodology based on mass spectrometry has been applied to both 4-hydroxynonenal and a variety of isoprostanes in human biological fluids. Measurement of these compounds in urine reflects lipid peroxidation in vivo and offers a noninvasive approach that may be readily applied to clinical trials. (C) 2000 Elsevier Science Inc.