Interaction of activated EGF receptors with coated pit adaptins.
Interaction of activated EGF receptors with coated pit adaptins.
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激活的 EGF 受体与包被的凹坑适应素的相互作用。
DOI：
10.1126/science.8342026
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Carpenter,G
中科院分区：
文献类型：
--
作者：
Sorkin,A;Carpenter,G
表皮生长因子 (EGF) 受体在胞吞过程中通过包被的凹坑与质膜相关的衔接蛋白相互作用。当 A-431 细胞在 37°C（而非 4°C）下用 EGF 处理时，几乎 50% 的 α-适应素总量与 EGF 受体发生共免疫沉淀。 α-适应素的部分蛋白水解表明氨基末端结构域是与 EGF 受体相关的区域。在缺乏钾以阻止内吞作用的细胞中，受体-适应素关联的程度增加。这些数据表明，在包被的小坑完全组装之前，完整细胞中就发生了受体-适应素的关联。
The epidermal growth factor (EGF) receptor interacts with plasma membrane-associated adapter proteins during endocytosis through coated pits. Almost 50 percent of the total pool of α-adaptins was coimmunoprecipitated with the EGF receptor when A-431 cells were treated with EGF at 37°C, but not at 4°C. Partial proteolysis of α-adaptin suggested that the amino-terminal domain is the region that associates with the EGF receptor. The extent of receptor-adaptin association was increased in cells depleted of potassium to block endocytosis. These data suggest that receptor-adaptin association occurs in intact cells before coated pits are fully assembled.