ISOLATION, DNA-SEQUENCE, AND REGULATION OF A MEIOSIS-SPECIFIC EUKARYOTIC RECOMBINATION GENE
ISOLATION, DNA-SEQUENCE, AND REGULATION OF A MEIOSIS-SPECIFIC EUKARYOTIC RECOMBINATION GENE
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DOI：
10.1073/pnas.84.22.8035
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发表时间：
1987-11-01
影响因子：
11.1
通讯作者：
ELDER, RT
中科院分区：
文献类型：
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作者：
ATCHESON, CL;DIDOMENICO, B;ELDER, RT
SPO 11基因，所需的减数分裂重组酿酒酵母，已被克隆的spo 11 -1表型的互补直接选择：缺乏减数分裂重组和低孢子活力。DNA测序表明，该基因编码398个氨基酸的蛋白质，预测分子量为45.3 kDa。SPO 11蛋白和其他蛋白序列之间没有显著的相似性，包括那些已知参与DNA重组或修复的基因。携带破坏等位基因的菌株是可行的，表明SPO 11是有丝分裂生长的抑制剂。RNA分析表明，SPO 11产生的1.5-脱氢酶的转录本，发育调控和孢子形成过程的早期表达。
The SPO11 gene, required for meiotic recombination in Saccharomyces cerevisiae, has been cloned by direct selection for complementation of the spo11-1 phenotype:lack of meiotic recombination and low spore viability. DNA sequencing indicates that the gene encodes a 398-amino acid protein having a predicted molecular mass of 45.3 kDa. There is no significant similarity between the SPO11 protein and other protein sequences, including those from genes known to be involved in DNA recombination or repair. Strains bearing a disruption allele are viable, indicating that SPO11 is dispensable for mitotic growth. RNA analyses demonstrate that SPO11 produces a 1.5-kilobase transcript that is developmentally regulated and expressed early in the sporulation process.