Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage
Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage
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DOI：
10.1073/pnas.93.14.7125
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发表时间：
1996-07-09
影响因子：
11.1
通讯作者：
Brown, WRA
中科院分区：
文献类型：
--
作者：
Heller, R;Brown, KE;Brown, WRA
我们已经使用端粒DNA将人类Y染色体的两个端着丝粒衍生物分解成小到可以通过脉冲场凝胶电泳进行大小分离的小染色体。其中一个小染色体的大小约为7Mb，对该分子的序列标记位点分析表明，它对应于Y染色体短臂的简单截断。其中5条微小染色体来源于长臂，全部经过一次简单的截断重新排列，大小从4.0Mb到9Mb不等。我们已经研究了这些小染色体的有丝分裂稳定性，并表明它们在培养中增殖约100个细胞分裂后保持稳定。
We have used telomeric DNA to break two acrocentric derivatives of the human Y chromosome into mini-chromosomes that are small enough to be size-fractionated by pulsed-field gel electrophoresis. One of the mini-chromosomes is about 7 Mb in size and sequence-tagged site analysis of this molecule suggests that it corresponds to a simple truncation of the short arm of the Y chromosome. Five of the mini-chromosomes are derived from the long arm, are all rearranged by more than a simple truncation, and range in size from 4.0 Mb to 9 Mb. We have studied the mitotic stabilities of these mini-chromosomes and shown that they are stably maintained by cells proliferating in culture for about 100 cell divisions.