The antibacterial innate immune response by the mosquito Aedes aegypti is mediated by hemocytes and independent of Gram type and pathogenicity
The antibacterial innate immune response by the mosquito Aedes aegypti is mediated by hemocytes and independent of Gram type and pathogenicity
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DOI：
10.1016/j.micinf.2004.01.005
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发表时间：
2004-04-01
影响因子：
5.8
通讯作者：
Christensen, BM
中科院分区：
文献类型：
--
作者：
Hillyer, JF;Schmidt, SL;Christensen, BM
以往的蚊虫研究表明，血细胞对革兰氏(-)大肠埃希菌的天然免疫反应是吞噬反应，而对革兰氏(+)微球菌的天然免疫反应是黑化反应。我们检测了埃及伊蚊对革兰氏(-)阴沟肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、鼠伤寒沙门氏菌、革兰氏(+)蜡状芽孢杆菌、粪肠球菌、金黄色葡萄球菌和表皮葡萄球菌的免疫应答。利用光学显微镜、电子显微镜和生存分析，这项研究最终表明，控制吞噬细胞对黑化反应的因素是复杂的，与细菌的革兰氏类型和致病性无关。这些数据进一步证明血细胞是针对原核生物的免疫反应的中心。(C)2004年爱思唯尔公司。版权所有。
Previous mosquito studies showed that the hemocyte-mediated innate immune response against Gram(-) Escherichia coli is phagocytosis, but against Gram(+) Micrococcus sp., is melanization. We examined the immune responses mounted by Aedes aegypti towards Gram(-) Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella typhimurium, and Gram(+) Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis. Using light microscopy, electron microscopy, and survival analysis, this investigation conclusively shows that the factors governing phagocytic vs. melanization responses are complex and independent of bacterial Gram type and pathogenicity. These data provide further evidence that hemocytes are central to the immune response against prokaryotes. (C) 2004 Elsevier SAS. All rights reserved.