Alterations of glutathione S-transferase and matrix metalloproteinase-9 expressions are early events in esophageal carcinogenesis
Alterations of glutathione S-transferase and matrix metalloproteinase-9 expressions are early events in esophageal carcinogenesis
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DOI：
10.3748/wjg.v13.i5.676
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发表时间：
2007-02-07
影响因子：
4.3
通讯作者：
Tulassay, Zsolt
中科院分区：
文献类型：
--
作者：
Herszenyi, Laszlo;Hritz, Istvan;Tulassay, Zsolt
目的：探讨谷胱甘肽S-转移酶（GST）和基质Meta蛋白酶-9（MMP-9）在食管反流性食管炎-Barrett's Meta-异型增生-腺癌序列发生发展中的作用。采用免疫组织化学方法检测了51例石蜡包埋组织中GST和MMP-9的表达，其中包括反流性食管炎患者（n = 7），Barrett化生（n = 14）、Barrett食管炎（n = 8）、Barrett食管异型增生（n = 7）、食管腺癌（n = 8）和无任何组织学变化的对照组（n = 7）。免疫染色半定量测定。统计分析采用单因素方差分析、LSD检验和相关分析。结果：与Barrett化生组及其他各组相比，对照组GST表达显著增高，MMP-9表达显著降低。Barrett食管炎和Barrett食管炎合并症之间未观察到重大变化。Barrett和伴随的异型增生，和腺癌显示GST的表达显着降低和MMP-9的水平高于所有其他组。腺癌几乎没有GST表达，MMP-9的水平明显高于Barrett和伴随的异型增生。GST和MMP-9的变化呈负相关（r = 0.82）。结论：Barrett's Meta-化生-腺癌序列中GST表达降低，MMP-9表达升高，与食管癌的发生有关。GST的损失和获得的MMP-9在巴雷特异型增生与非异型增生化生相比，表明这些变化可能是早期事件在癌变。Barrett食管中这些参数的定量可能有助于识别癌症进展风险较高的患者。(c)2007年，WJG出版社。All rights reserved.
AIM: To investigate the role of glutathione S-transferase (GST) and matrix meta lloproteinase-9 (MMP-9) expressions in the development and progression of reflux esophagitis-Barrett's meta plasia-dysplasia-adenocarcinoma sequence in the esophagus.METHODS: GST and MMP-9 expressions were analyzed in 51 paraffin-embedded tissue samples by immunohistochemistry including patients with reflux esophagitis (n = 7), Barrett's metaplasia (n = 14), Barrett and esophagitis (n = 8), Barrett and dysplasia (n = 7), esophageal adenocarcinoma (n = 8) and a control group without any histological changes (n = 7). Immunostaining was determined semiquantitatively. Statistical analysis with one-way ANOVA, LSD test and correlation analysis were performed. P value of < 0.05 was considered significant.RESULTS: GST expression was significantly higher while MMP-9 expression was significantly lower in control group compared to Barrett's metaplasia and the other groups. No major changes were observed between Barrett, esophagitis, and Barrett and concomitant esophagitis. Barrett and concomitant dysplasia, and adenocardnoma revealed a significant lower expression of GST and higher levels of MMP-9 compared to all other groups. Adenocarcinoma showed almost no expression of GST and significantly higher levels of MMP-9 than Barrett and concomitant dysplasia. Alterations of GST and MMP-9 were inversely correlated (r 0.82).CONCLUSION: Decreased GST and increased expression of MMP-9 in Barrett's meta plasia-clysplasiaadenocarcinoma sequence as compared to normal tissue suggest their association with esophageal tumorigenesis. Loss of GST and gain of MMP-9 in Barrett with dysplasia compared to non-clysplastic metaplasia indicate that these alterations may be early events in carcinogenesis. Quantification of these parameters in Barrett's esophagus might be useful to identify patients at higher risk for progression to cancer. (c) 2007 The WJG Press. All rights reserved.