PROPERTIES OF SACCHAROMYCES-CEREVISIAE WEE1 AND ITS DIFFERENTIAL REGULATION OF P34(CDC28) IN RESPONSE TO G(1) AND G(2) CYCLINS
PROPERTIES OF SACCHAROMYCES-CEREVISIAE WEE1 AND ITS DIFFERENTIAL REGULATION OF P34(CDC28) IN RESPONSE TO G(1) AND G(2) CYCLINS
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DOI：
10.1002/j.1460-2075.1993.tb06016.x
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发表时间：
1993-09-01
期刊：
影响因子：
11.4
通讯作者：
KIRSCHNER, MW
中科院分区：
文献类型：
--
作者：
BOOHER, RN;DESHAIES, RJ;KIRSCHNER, MW
Wee1 是一种蛋白激酶，可负向调节 p34cdc2 激酶活性。我们已经鉴定出由 SWE1 基因编码的酿酒酵母 wee1 同源物。 SWE1 过表达使细胞停滞在具有短纺锤体的 G2 期，而删除 SWE1 不会改变细胞周期，但确实消除了 mih1- 突变体中观察到的 G2 延迟。 Swe1 免疫沉淀物能够酪氨酸磷酸化和灭活与 Clb2（一种 G2 型细胞周期蛋白）复合的 p34CDC28，但不能使与 Cln2（一种 G1 型细胞周期蛋白）复合的 p34CDC28 失活，这与 Swe1 过表达无法抑制 G1/S 转变一致。这些结果表明，特定的细胞周期蛋白亚基针对 p34CDC28 进行不同的调节控制，这对于确保细胞周期中 p34CDC28 的正常功能可能很重要。
Wee1 is a protein kinase that negatively regulates p34cdc2 kinase activity. We have identified a Saccharomyces cerevisiae wee1 homolog encoded by the SWE1 gene. SWE1 overexpression arrests cells in G2 with short spindles whereas deletion of SWE1 did not alter the cell cycle but did eliminate the G2 delay observed in mih1- mutants. Swe1 immunoprecipitates were capable of tyrosine phosphorylating and inactivating p34CDC28 complexed with Clb2, a G2-type cyclin, but not p34CDC28 complexed with Cln2, a G1-type cyclin, consistent with the inability of Swe1 overexpression to inhibit the G1/S transition. These results suggest that specific cyclin subunits target p34CDC28 for distinct regulatory controls which may be important for ensuring proper p34CDC28 function during the cell cycle.