Leaf form diversification in an ornamental heirloom tomato results from alterations in two different HOMEOBOX genes
Leaf form diversification in an ornamental heirloom tomato results from alterations in two different HOMEOBOX genes
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DOI：
10.1016/j.cub.2021.08.023
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发表时间：
2021-11-08
期刊：
影响因子：
9.2
通讯作者：
Sinha, Neelima R.
中科院分区：
文献类型：
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作者：
Nakayama, Hokuto;Rowland, Steven D.;Sinha, Neelima R.
驯化植物表现出不同的表型特征。然而，育种努力对这种表型多样性的影响仍不清楚。在这里，我们证明了BIPINNAA同源框基序的单核苷酸缺失，这是一个类似BEL的同源结构域基因，导致了作为园林和观赏植物的番茄(Solanum Lycopsicum)，银杉(SIFT)的高度复杂的叶片表型。比较基因网络分析表明，SIFT中出现的窄叶表型是由于WUSCHEL相关同源框1的同源同源基因SOLANIFOLIA的抑制造成的。比较基因组学表明，SIFT中的bip突变可能是从头开始的，是SIFT所特有的，而不是从其他番茄基因组中导入的。这些结果为理解在驯化后的改良过程中引入作物的表型性状的自然变异提供了新的见解，并建立了同源框基因作为进化热点。
Domesticated plants display diverse phenotypic traits. However, the influence of breeding effort on this phenotypic diversity remains unknown. Here, we demonstrate that a single nucleotide deletion in the homeobox motif of BIPINNATA, a BEL-LIKE HOMEODOMAIN gene, led to a highly complex leaf phenotype in an heirloom tomato (Solanum lycopersicum), Silvery Fir Tree (SiFT), which is used as a landscaping and ornamental plant. A comparative gene network analysis revealed that repression of SOLANIFOLIA, the ortholog of WUSCHEL RELATED HOMEOBOX 1, caused the narrow leaflet phenotype seen in SiFT. Comparative genomics indicated that the bip mutation in SiFT likely arose de novo and is unique to SiFT and not introgressed from other tomato genomes. These results provide new insights into the natural variation in phenotypic traits introduced into crops during improvement processes after domestication and establish homeobox genes as evolutionary hotspots.