Polyethylene sulfonate: a tight-binding inhibitor of 6-phosphogluconate dehydrogenase of Cryptococcus neoformans.
Polyethylene sulfonate: a tight-binding inhibitor of 6-phosphogluconate dehydrogenase of Cryptococcus neoformans.
复制标题
聚乙烯磺酸盐：新型隐球菌 6-磷酸葡萄糖酸脱氢酶的紧密结合抑制剂。
DOI：
10.1006/abbi.1995.0045
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发表时间：
1995
影响因子：
3.9
通讯作者：
Ray,WK
中科院分区：
文献类型：
--
作者：
Niehaus,WG;White,RH;Richardson,SB;Bourne,A;Ray,WK
Polyethylene sulfonate (PES) or polyvinyl sulfonate was found to be a potent inhibitor of a number of fungal enzymes, including 6-phosphogluconate dehydrogenase fromCryptococcus neoformans.The inhibition was apparently competitive versus either NADP or 6-phosphogluconate, with 50% inhibition at PES concentrations below 10 nM. Replots of slopes of double-reciprocal plots versus inhibitor concentration were sharply concave upward, whereas replots of slope versus [PES]3were linear. The inhibition was freely reversible upon dilution of the enzyme–PES complex. A model is presented that involves initial binding of the long (Mr50,000) polyanionic PES at a remote site on the enzyme, followed by interaction of the end of the tethered polymer with the binding site for NADP or for 6-phosphogluconate.
