Crystallization Technique for Strain-free Protein Crystals Using Cross-linked Seed Crystals
Crystallization Technique for Strain-free Protein Crystals Using Cross-linked Seed Crystals
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DOI：
10.1021/acs.cgd.6b01136
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发表时间：
2016-09
影响因子：
3.8
通讯作者：
H. Koizumi;S. Uda;M. Tachibana;K. Tsukamoto;K. Kojima;J. Nozawa
中科院分区：
文献类型：
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作者：
H. Koizumi;S. Uda;M. Tachibana;K. Tsukamoto;K. Kojima;J. Nozawa
在这项研究中，我们通过x射线衍射（XRD）岩石曲线测量表明，利用与戊二醛交联的种子晶体可以在很大程度上去除四方蛋清（HEW）溶菌酶晶体中的局部应变。我们还发现，当采用交联种子晶体时，亚晶粒和亚晶粒之间的取向偏差减小，这表明使用这种技术可以控制四方HEW溶菌酶晶体的亚晶粒形成。以四边形HEW溶菌酶晶体为模型蛋白，讨论了理想的蛋白质镶嵌结构，使其能够收集精确的衍射波积分强度。
In this study, we show using X-ray diffraction (XRD) rocking-curve measurements that local strain in tetragonal hen egg white (HEW) lysozyme crystals can be removed to a large degree by utilizing seed crystals cross-linked with glutaraldehyde. We also find that misorientation between subgrains and subgrain size decrease when employing cross-linked seed crystals, which suggests that subgrain formation in tetragonal HEW lysozyme crystals can be controlled using this technique. The ideal mosaic structure of proteins that enables the collection of accurate integrated intensities of the diffracted waves is also discussed using tetragonal HEW lysozyme crystals as a model protein.