Influence of cation adduction on the separation characteristics of flavonoid diglycoside isomers using dual gate-ion mobility-quadrupole ion trap mass spectrometry
Influence of cation adduction on the separation characteristics of flavonoid diglycoside isomers using dual gate-ion mobility-quadrupole ion trap mass spectrometry
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DOI：
10.1002/jms.994
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Hill, HH
中科院分区：
文献类型：
--
作者：
Clowers, BH;Hill, HH
用电喷雾电离-双栅离子迁移率-四极杆离子陷阱质谱仪研究了钠、钾、银加成的异构体黄酮二糖苷的分离特性。这种仪器配置允许离子在质量分析之前根据它们的气相构象选择性地积累在离子陷阱内。对于所考察的金属阳离子，银与黄酮二糖苷形成了最紧密的加合物。按大小顺序排列，黄酮二糖苷与Na+、K+、Ag+加合的离子中性截面趋势为：柚皮苷、橙皮苷、新橙皮苷。为了考察糖基对分离的贡献，将橙皮苷、橙皮苷的苷元和新橙皮苷与芦丁的苷元槲皮素进行了比较。黄酮类二苷类化合物的分离表明，与橙皮素衍生的异构体相比，槲皮素衍生的二苷类化合物漂移更长。结合观察到的碰撞辅助解离(CAD)数据，这些发现表明碳水化合物部分在黄酮二糖苷的分离和金属螯合特性中起着重要作用。版权所有(C)2006 John Wiley&Sons，Ltd.
An electrospray ionization-dual gate-ion mobility-quadrupole ion trap mass spectrometer was used to evaluate the separation characteristics of isomeric flavonoid diglycosides adducted with sodium, potassium, and silver. This instrumental configuration allows ions to be selectively accumulated within the ion trap on the basis of their gas phase conformation prior to mass analysis. For the metal cations examined, silver produced the most compact adducts with flavonoid diglycosides. Listed in order of increasing size, the trend of flavonoid diglycoside ion-neutral cross sections adducted with Na+, K+, and Ag+ was narirutin < naringin < hesperidin < neohesperidin < rutin. To examine the separation contribution of the carbohydrate group, hesperetin, the aglycone of hesperidin, and neohesperin were compared to quercetin, the aglycone of rutin. Separation of the flavonoid diglycosides indicated that quercetin-derived diglycosides drifted longer than their hesperetin-derived isomers. Combined with the observed collision assisted dissociation (CAD) data, these findings suggest that carbohydrate moiety plays a significant role in both the separation and metal chelating characteristics of flavonoid diglycosides. Copyright (c) 2006 John Wiley & Sons, Ltd.