High ionic strength glucose-sensing photonic crystal
High ionic strength glucose-sensing photonic crystal
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DOI：
10.1021/ac030021m
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发表时间：
2003-05-15
影响因子：
7.4
通讯作者：
Asher, SA
中科院分区：
文献类型：
--
作者：
Alexeev, VL;Sharma, AC;Asher, SA
我们展示了一种比色葡萄糖识别材料，该材料由嵌入在聚丙烯酰胺-聚乙二醇（PEG）水凝胶或聚丙烯酰胺-15-冠-5水凝胶中的晶体胶体阵列组成，具有悬垂的苯硼酸基团。我们利用了一种新的分子识别基序，其中硼酸和PEG（或冠醚）官能团预先定位在光子晶体水凝胶中，这样葡萄糖将这些官能团自组装成一个超分子复合物。复合物的形成导致水凝胶交联的增加，这对于生理上相关的葡萄糖浓度蓝移光子晶体衍射。视觉上明显的衍射颜色在生理上重要的葡萄糖浓度范围内跨越可见光谱区域。这些材料在生理离子强度和pH值下对葡萄糖产生反应，并且对葡萄糖的反应模式比半乳糖、甘露糖和果糖有选择性。因此，我们开发了一种新的葡萄糖识别基序，有望为糖尿病患者制造无创或微创体内葡萄糖传感。
We demonstrate a colorimetric glucose recognition material consisting of a crystalline colloidal array embedded within a polyacrylamide-poly(ethylene glycol) (PEG) hydrogel, or a polyacrylamide-15-crown-5 hydrogel, with pendent phenylboronic acid groups. We utilize a new molecular recognition motif, in which boronic acid and PEG (or crown ether) functional groups are prepositioned in a photonic crystal hydrogel, such that glucose self-assembles these functional groups into a supramolecular complex. The formation of the complex results in an increase in the hydrogel cross-linking, which for physiologically relevant glucose concentration blue shifts the photonic crystal diffiraction. The visually evident diffraction color shifts across the visible spectral region over physiologically important glucose concentration ranges. These materials respond to glucose at physiological ionic strengths and pH values and are selective in their mode of response for glucose over galactose, mannose, and fructose. Thus, we have developed a new recognition motif for glucose that shows promise for the fabrication of noninvasive or minimally invasive in vivo glucose sensing for patients with diabetes mellitus.