Parvalbumin, somatostatin and cholecystokinin as chemical markers for specific GABAergic interneuron types in the rat frontal cortex
Parvalbumin, somatostatin and cholecystokinin as chemical markers for specific GABAergic interneuron types in the rat frontal cortex
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DOI：
10.1023/a:1024126110356
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发表时间：
2002-03-01
期刊：
影响因子：
--
通讯作者：
Kondo, S
中科院分区：
文献类型：
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作者：
Kawaguchi, Y;Kondo, S
皮质回路中存在多少类GABA能非锥体细胞仍有待澄清。我们将大鼠额叶皮层的GABA细胞分为3组，根据它们的放电特征：快峰（FS）细胞，晚峰（LS）细胞和非FS细胞。钙结合蛋白和肽的表达可在额叶皮质的II/III和V层中的不同GABA细胞群中显示：（1）小白蛋白细胞，（2）生长抑素细胞，（3）钙视网膜蛋白和/或血管活性肠多肽（VIP）细胞[胆囊收缩素（CCK）部分阳性]和（4）大CCK细胞（VIP/钙视网膜蛋白几乎阴性）。结合形态多样的非锥体细胞的生理和化学特性，可以分为几组，包括FS篮状细胞含有小清蛋白，非FS生长抑素Martinotti细胞与上行轴突乔木，和非FS大篮状细胞CCK阳性。这些亚型显示了轴突侧支的特征性空间分布和突触后成分的神经支配倾向。与皮层兴奋性或抑制性回路引起的同步活动，放电模式也被发现不同。亚型选择性发生的电耦合，发现钾通道Kv3.1蛋白，胆碱能和胆碱能调制支持我们的初步分类。为了阐明额叶皮层的功能结构，重要的是要揭示GABA细胞亚型的连接特征，并确定它们是否与其他皮层区域相似。
It remains to be clarified how many classes of GABAergic nonpyramidal cells exist in the cortical circuit. We have divided GABA cells in the rat frontal cortex into 3 groups, based on their firing characteristics: fast-spiking (FS) cells, late-spiking (LS) cells, and non-FS cells. Expression of calcium-binding proteins and peptides could be shown in separate groups of GABA cells in layers II/III and V of the frontal cortex: (1) parvalbumin cells, (2) somatostatin cells, (3) calretinin and/or vasoactive intestinal polypeptide (VIP) cells [partially positive for cholecystokinin (CCK)] and (4) large CCK cells (almost negative for VIP/calretinin). Combining the physiological and chemical properties of morphologically diverse nonpyramidal cells allows division into several groups, including FS basket cells containing parvalbumin, non-FS somatostatin Martinotti cells with ascending axonal arbors, and non-FS large basket cells positive for CCK. These subtypes show characteristic spatial distributions of axon collaterals and the innervation tendency of postsynaptic elements. With synchronized activity induced by cortical excitatory or inhibitory circuits, firing patterns were also found to differ. Subtype-selective occurrence of electrical coupling, finding for potassium channel Kv3.1 proteins, and cholinergic and serotonergic modulation supports our tentative classification. To clarify the functional architecture in the frontal cortex, it is important to reveal the connectional characteristics of GABA cell subtypes and determine whether they are similar to those in other cortical regions.