Synergy of lignocelluloses pretreatment by sodium carbonate and bacterium to enhance enzymatic hydrolysis of rice straw.
Synergy of lignocelluloses pretreatment by sodium carbonate and bacterium to enhance enzymatic hydrolysis of rice straw.
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DOI：
10.1016/j.biortech.2017.10.008
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发表时间：
2018-02
影响因子：
11.4
通讯作者：
Zhanhui Shen;Kejing Zhang;Mengying Si;Mingren Liu;S. Zhuo;Dan Liu;Lili Ren;Xu Yan;Yan Shi
中科院分区：
文献类型：
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作者：
Zhanhui Shen;Kejing Zhang;Mengying Si;Mingren Liu;S. Zhuo;Dan Liu;Lili Ren;Xu Yan;Yan Shi
研究了一种在温和条件下提高水稻秸秆酶解效率的预处理新策略。这种方法使用碳酸钠（Na2CO3）和菌株Cupriavidus basilensis B-8（以下简称B-8）的协同作用。na2co3和B-8协同预处理（SNBP）后，不同条件下的还原糖产率在335.3 ~ 799.6 mg/g之间变化。这比na2co3预处理（284.2-719.2 mg/g）增加了13-31%，比未处理的RS （98 mg/g）增加了3.42-8.15倍。此外，通过木质素和半纤维素的减少，RS的组成发生了显著变化。我们通过对预处理前后RS结构的成分分析和理化表征证实了这一变化。我们还阐述了SNBP的机制，以更好地解释RS的变化和细菌对酶水解的影响。
We studied a new strategy for pretreatment of rice straw (RS) to enhance enzymatic hydrolysis under mild condition. This approach uses the synergy of sodium carbonate (Na2CO3) and the bacterial strain Cupriavidus basilensis B-8 (hereafter B-8). After synergistic Na2CO3and B-8 pretreatment (SNBP), the reducing sugar yield varied from 335.3 mg/g to 799.6 mg/g under different conditions. This increased by 13–31% over Na2CO3pretreatment (284.2–719.2 mg/g) and 3.42–8.15 times over the untreated RS (98 mg/g). Moreover, the composition of RS was changed significantly through decreases in lignin and hemicellulose. We confirmed this change by compositional analysis and physicochemical characterization of the structure of RS before and after pretreatment. We also elaborated a mechanism for SNBP to better explain RS changes and bacterial effects on enzymatic hydrolysis.