GENOMIC DIVERSITY AND ORGANIZATION OF VIRULENCE GENES IN THE PATHOGENIC ANAEROBE CLOSTRIDIUM-PERFRINGENS
GENOMIC DIVERSITY AND ORGANIZATION OF VIRULENCE GENES IN THE PATHOGENIC ANAEROBE CLOSTRIDIUM-PERFRINGENS
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DOI：
10.1111/j.1365-2958.1992.tb00862.x
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发表时间：
1992-06-01
影响因子：
3.6
通讯作者：
COLE, ST
中科院分区：
文献类型：
--
作者：
CANARD, B;SAINTJOANIS, B;COLE, ST
脉冲场凝胶电泳已用于评估基因组多样性和鉴定10株菌株的毒力区域，代表厌氧病原体产气荚膜梭菌的所有五种血清型。在8个病例中建立了几乎等于3.6 Mb的圆形染色体的详细物理和基因图谱，并用于推导共识图谱。除了一个例外，染色体排列是相对恒定的，地图比较允许三个高可变区域被确定。其中之一与肠毒素基因（cpe）有关，这是在摄入受产气荚膜荚膜杆菌污染的食物后导致人类腹泻的一个重要原因。另一个跨越主要毒力基因plc的可变区域，编码细胞溶解毒素α，在所有病例中都位于oriC附近，而另一个致命分型毒素epsilon的基因则由一个片段携带。现在看来，构成产气荚膜梭菌分型系统的血清学变异和致病谱的变化可能完全是由于染色体外遗传因素的丧失或获得。
Pulsed-field gel electrophoresis has been used to assess genomic diversity and to identify virulence regions in 10 strains, representing all five serotypes, of the anaerobic pathogen Clostridium perfringens. Detailed physical and gene maps of the almost-equal-to 3.6 Mb circular chromosomes have been established in eight cases and used to deduce a consensus map. With one exception the chromosomal arrangement was relatively constant and map comparison allowed three hypervariable regions to be identified. One of these was associated with the enterotoxin gene, cpe, which is an important cause of human diarrhoea following the ingestion of food contaminated with C. perfringens. Another variable region spanning the major virulence gene plc, which encodes the cytolytic toxin, alpha, was located near oriC in all cases whereas the gene for another lethal typing toxin, epsilon, was borne by an episome. It now seems likely that the serological variations, and the changes in the pathogenic spectrum which constitute the C. perfringens typing system, may be due entirely to the loss, or acquisition, of extrachromosomal genetic elements.