Hippocampal networks on reliable patterned substrates.
Hippocampal networks on reliable patterned substrates.
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DOI：
10.1016/j.jneumeth.2011.09.020
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发表时间：
2012-01-30
影响因子：
3
通讯作者：
Brewer, Gregory J.
中科院分区：
文献类型：
--
作者：
Boehler, Michael D.;Leondopulos, Stathis S.;Wheeler, Bruce C.;Brewer, Gregory J.
为了实现可复制的活神经元网络，我们研究了底物模式对神经元顺应性和网络活动的影响。我们优化了微接触印刷的工艺参数，以在多电极阵列（MEA）的记录电极上的恒定方形节点阵列上，以4、6或8个连接的10µm宽的聚赖氨酸线可重复的几何图案。我们假设节点连接的增加会给网络更多的输入，从而导致更高的神经元输出，作为网络峰值速率。我们还在发育过程中长期刺激这些网络，并添加星形胶质细胞来增强网络活动。我们的研究结果表明，尽管神经元体在电极上频繁定位，但与随机网络相比，自发活动的电极数量减少了3倍，与模式复杂性无关。在激活的电极中，总体峰值速率与模式复杂性无关，与活动的动态平衡一致。模式复杂度越高，平均爆发率越低；然而，随着模式复杂性的增加，爆发持续时间增加了1.6倍（n=6027次爆发，p<0.001）。脉冲间隔和显示脉冲的活性电极的百分比也随着模式复杂性的增加而增加。随着模式复杂性的增加，额外爆发（非爆发或孤立的）尖峰率增加了4倍，但这种关系在慢性刺激或星形胶质细胞增加时是相反的。这些研究首次表明，限制分支和输入分布的模式对海马体网络的活动是有害的，但更高水平的模式复杂性促进了非爆发活动，并有利于持续更长的时间，但较少的爆发。
Toward the goal of reproducible live neuronal networks, we investigated the influence of substrate patterns on neuron compliance and network activity. We optimized process parameters of micro-contact printing for reproducible geometric patterns of 10 µm wide lines of polylysine with 4, 6, or 8 connections at a constant square array of nodes overlying the recording electrodes of a multielectrode array (MEA). We hypothesized that an increase in node connections would give the network more inputs resulting in higher neuronal outputs as network spike rates. We also chronically stimulated these networks during development and added astroglia to enhance network activity. Our results show that despite frequent localization of neuron somata over the electrodes, the number of spontaneously active electrodes was reduced 3-fold compared to random networks, independent of pattern complexity. Of the electrodes active, the overall spike rate was independent of pattern complexity, consistent with homeostasis of activity. Lower mean burst rates were seen with higher levels of pattern complexity; however, burst durations increased 1.6-fold with pattern complexity (n=6027 bursts, p<0.001). Inter-burst interval and percentage of active electrodes displaying bursts also increased with pattern complexity. The extra-burst (non-burst or isolated) spike rate increased 4-fold with pattern complexity, but this relationship was reversed with either chronic stimulation or astroglia addition. These studies suggest for the first time that patterns which limit the distribution of branches and inputs are deleterious to activity in a hippocampal network, but that higher levels of pattern complexity promote non-burst activity and favor longer lasting, but fewer bursts.
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影响因子：
14
作者：
Branch, DW;Wheeler, BC;Leckband, DE
通讯作者：
Leckband, DE
影响因子：
25
作者：
Clopath, Claudia;Buesing, Lars;Gerstner, Wulfram
通讯作者：
Gerstner, Wulfram
影响因子：
--
作者：
Boehler, Michael D.;Wheeler, Bruce C.;Brewer, Gregory J.
通讯作者：
Brewer, Gregory J.
影响因子：
5.3
作者：
Maffei, Arianna;Turrigiano, Gina G.
通讯作者：
Turrigiano, Gina G.
影响因子：
3.4
作者：
Matsuzawa, M;Tabata, T;Kano, M
通讯作者：
Kano, M