Fluorescent hydrogen peroxide sensor based on cupric oxide nanoparticles and its application for glucose and L-lactate detection
Fluorescent hydrogen peroxide sensor based on cupric oxide nanoparticles and its application for glucose and L-lactate detection
复制标题
基于氧化铜纳米颗粒的荧光过氧化氢传感器及其在葡萄糖和L-乳酸检测中的应用
DOI：
10.1016/j.bios.2014.05.048
复制
发表时间：
2014-11-15
影响因子：
12.6
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Hu, Ai-Ling;Liu, Yin-Huan;Chen, Wei
基于纳米氧化铜的类过氧化物酶活性，研制了一种新型的过氧化氢荧光传感器。氧化铜纳米粒子有效地催化过氧化氢分解为羟基自由基。然后用羟基自由基氧化对苯二甲酸，生成高荧光产物。过氧化氢的线性范围估计为5.0 x 10（-6）-2.0 x 10（-4）M，检测限为3.4 x 10（-7）M。此外，该检测系统能够感测可以酶促产生过氧化氢的分析物。通过将葡萄糖或L-乳酸的氧化酶催化氧化与氧化铜纳米颗粒催化的对苯二甲酸氧化偶联，实现了葡萄糖和L-乳酸的灵敏测定，检测限分别为1.0 × 10 ~（-6）和4.5 × 10 ~（-8）M。这种方法在人血清样品中的成功应用也得到了证实。(C)2014爱思唯尔有限公司版权所有。
A novel fluorescent hydrogen peroxide sensor was developed based on the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals. Then terephthalic acid was oxidized by hydroxyl radical to form a highly fluorescent product. The linear range of hydrogen peroxide estimated to be 5.0 x 10(-6)-2.0 x 10(-4) M with a detection limit of 3.4 x 10(-7) M. Moreover, this detection system enabled the sensing of analytes which can enzymatically generate hydrogen peroxide. By coupling the oxidation of glucose or L-lactate catalyzed by their corresponding oxidase enzymes with terephthalic acid oxidation catalyzed by cupric oxide nanoparticles, sensitive assays of glucose and L-lactate with detection limits of 1.0 x 10(-6) and 4.5 x 10(-8) M were realized. The successful applications of this approach in human serum samples have also been demonstrated. (C) 2014 Elsevier B.V. All rights reserved.