Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA
Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA
复制标题
塞姆利基森林病毒的复制：正链RNA中的聚腺苷酸和负链RNA中的聚尿苷酸
DOI：
10.1128/jvi.20.2.446-464.1976
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发表时间：
1976
影响因子：
5.4
通讯作者：
P. Gomatos
中科院分区：
文献类型：
--
作者：
D. Sawicki;P. Gomatos
来自塞姆利基森林病毒的42 S RNA含有80至90个残基长的聚腺苷酸[poly（A）]序列，并且是病毒粒子RNA的3 '末端。在感染后2 h分离的复制型（RF）和复制中间体（RI）的正链中发现了相同长度的poly（A）序列。此外，RF和RI均含有多聚尿苷酸[poly（U）]序列。在病毒体RNA中没有发现poly（U），因此poly（U）序列在负链RNA中。来自RF的poly（U）平均为60个残基长，而从RI分离的poly（U）为80个残基长。用RNase T1消化从RF和RI中分离的Poly（U）序列含有5 '-磷酸化的pUp和ppUp残基，表明poly（U）序列是负链RNA的5'-末端。RF或RI中的poly（U）序列仅在RNA变性后才能自由结合poly（A）-Sepharose，表明poly（U）与这些分子中正链RNA的3 '末端的poly（A）氢键结合。当用每毫升0.02 μ g RNase A处理时，RF和RI都产生了相同的三个核心分布，RFI，RFII和RFIII。RFI和RFIII的负链RNA均含有poly（U）序列。RFII没有。已知RFI是42 S正链RNA的双链形式，RFIII是实验衍生的26 S mRNA的双链形式。每一个中的poly（A）序列最有可能直接从42 S负链RNA的5 '端的poly（U）转录。因此，26 S mRNA代表包括其3 '末端的42 S正链RNA的三分之一中的核苷酸序列。
The 42S RNA from Semliki Forest virus contains a polyadenylate [poly(A)] sequence that is 80 to 90 residues long and is the 3'-terminus of the virion RNA. A poly(A) sequence of the same length was found in the plus strand of the replicative forms (RFs) and replicative intermediates (RIs) isolated 2 h after infection. In addition, both RFs and RIs contained a polyuridylate [poly(U)] sequence. No poly(U) was found in virion RNA, and thus the poly(U) sequence is in minus-strand RNA. The poly(U) from RFs was on the average 60 residues long, whereas that isolated from the RIs was 80 residues long. Poly(U) sequences isolated from RFs and RIs by digestion with RNase T1 contained 5'-phosphorylated pUp and ppUp residues, indicating that the poly(U) sequence was the 5'-terminus of the minus-strand RNA. The poly(U) sequence in RFs or RIs was free to bind to poly(A)-Sepharose only after denaturation of the RNAs, indicating that the poly(U) was hydrogen bonded to the poly(A) at the 3'-terminus of the plus-strand RNA in these molecules. When treated with 0.02 mug of RNase A per ml, both RFs and RIs yielded the same distribution of the three cores, RFI, RFII, and RFIII. The minus-strand RNA of both RFI and RFIII contained a poly(U) sequence. That from RFII did not. It is known that RFI is the double-stranded form of the 42S plus-strand RNA and that RFIII is the experimetnally derived double-stranded form of 26S mRNA. The poly(A) sequences in each are most likely transcribed directly from the poly(U) at the 5'-end of the 42S minus-strand RNA. The 26S mRNA thus represents the nucleotide sequence in that one-third of the 42S plus-strand RNA that includes its 3'-terminus.