Immunocytochemical investigation of immune cells within human primary and permanent tooth pulp
Immunocytochemical investigation of immune cells within human primary and permanent tooth pulp
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DOI：
10.1111/j.1365-263x.2006.00682.x
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发表时间：
2006-01-01
影响因子：
3.8
通讯作者：
Boissonade, F. M.
中科院分区：
文献类型：
--
作者：
Rodd, H. D.;Boissonade, F. M.
目的。本研究的目的是确定健康和疾病状态下人类乳牙和恒牙牙髓内免疫细胞的数量和分布是否存在差异。该研究采用定量免疫细胞化学研究的形式。在全身麻醉下从需要拔牙的儿童身上获取了一百二十四颗下颌第一恒磨牙和第二乳磨牙。拔牙后，对 10 微米厚的冷冻牙髓切片进行间接免疫荧光处理。采用以下组合的三重标记方案：(1)蛋白质基因产物9.5，一种通用神经元标记； (2)白细胞共同抗原(LCA)； (3) Ulex europaeus I 凝集素，血管内皮细胞的标记物。然后使用图像分析来确定 LCA 免疫染色的面积百分比。结果。与恒牙相比，完整和龋齿乳牙的牙髓角和冠状中部区域中的白细胞明显更丰富（P < 0.05，方差分析）。两个牙列都显示出存在良好的局部炎性细胞浸润，并且在白细胞密集积聚区域存在明显的牙髓神经坏死。结论。乳牙牙髓和恒牙牙髓似乎对粗龋具有相似的引发炎症反应的潜力。根据这些发现，需要重新评估受损乳牙牙髓的管理。
Aim. The aim of this study was to determine whether there are any differences in the number and distribution of immune cells within human primary and permanent tooth pulp, both in health and disease.Design. The research took the form of a quantitative immunocytochemical study. One hundred and twenty-four mandibular first permanent molars and second primary molars were obtained from children requiring dental extractions under general anaesthesia. Following exodontia, 10-mu m-thick frozen pulp sections were processed for indirect immunofluorescence. Triple-labelling regimes were employed using combinations of the following: (1) protein gene product 9.5, a general neuronal marker; (2) leucocyte common antigen (LCA); and (3) Ulex europaeus I lectin, a marker of vascular endothelium. Image analysis was then used to determine the percentage area of immunostaining for LCA.Results. Leucocytes were significantly more abundant in the pulp horn and mid-coronal region of intact and carious primary teeth, as compared to permanent teeth (P < 0.05, ANOVA). Both dentitions demonstrated the presence of well-localized inflammatory cell infiltrates and marked aborization of pulpal nerves in areas of dense leucocyte accumulation.Conclusions. Primary and permanent tooth pulps appear to have a similar potential to mount inflammatory responses to gross caries The management of the compromised primary tooth pulp needs to be reappraised in the light of these findings.