Production of diploid gynogenetic grass carp and triploid hybrids derived from the distant hybridization of female grass carp and male topmouth culter
Production of diploid gynogenetic grass carp and triploid hybrids derived from the distant hybridization of female grass carp and male topmouth culter
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雌性草鱼与雄性顶口鲌远缘杂交生产二倍体雌核草鱼和三倍体杂种
DOI：
10.1016/j.aquaculture.2018.12.056
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发表时间：
2019-04-15
期刊：
影响因子：
4.5
通讯作者：
Liu, Shaojun
中科院分区：
文献类型：
--
作者：
Wu, Chang;Huang, Xu;Liu, Shaojun
远缘杂交是创造不同倍性后代的有效手段，广泛应用于植物和低等生物。本研究通过远缘杂交，成功获得了雌性草鱼（Ctenopharyngodon idellus， GC, Leuciscinae, 2n = 48）与雄性上口鱼（Erythroculter ilishaeformis， TC, Cultrinae, 2n = 48）的二倍体雌性草鱼（2nGGC, 2n = 48）和三倍体杂交（3nGT, 3n = 72）后代。染色体数目、DNA含量、5S rDNA和荧光原位杂交（FISH）结果表明，2nGGC来源于48条染色体的雌核发生，不是GC和TC的杂种，而3nGT是72条染色体的三倍体杂种。3nGT与GC和TC表型差异显著，而2nGGC与GC表型相似。在摄食方面，2nGGC和3nGT均为草食性。2nGGC和3nGT组肌肉中总氨基酸含量极显著高于GC组（P < 0.01）。这是通过GC和TC杂交产生的2nGGC和3nGT的首次报道。两种新子代的形成在鱼类遗传育种中具有十分重要的意义。
Distant hybridization is an effective tool to create offspring with different ploidy levels and is widely used in plants and lower organisms. In this study, the diploid gynogenetic grass carp (2nGGC, 2n = 48) and triploid hybrid (3nGT, 3n = 72) progenies of female grass carp (Ctenopharyngodon idellus, GC, Leuciscinae, 2n = 48) x male topmouth culter (Erythroculter ilishaeformis, TC, Cultrinae, 2n = 48) were successfully obtained by distant hybridization. The results regarding the chromosomal number, DNA content, 5S rDNA and fluorescence in situ hybridization (FISH) showed 2nGGC was derived from gynogenesis with 48 chromosomes and not a hybrid of GC and TC, while 3nGT was a triploid hybrid with 72 chromosomes. 3nGT exhibited significant phenotypic differences from GC and TC, but 2nGGC resembled GC. With regard to feeding, both 2nGGC and 3nGT were herbivorous. However, the total amino acid contents in the muscles of both 2nGGC and 3nGT were significantly higher than that in GC (P < 0.01). This is the first report of 2nGGC and 3nGT being produced by crossing GC and TC. The formation of two kinds of new offspring is very important in fish genetic breeding.