microRNA-dependent gene regulatory networks in maize leaf senescence.
microRNA-dependent gene regulatory networks in maize leaf senescence.
复制标题
玉米叶片衰老中 microRNA 依赖性基因调控网络
DOI：
10.1186/s12870-016-0755-y
复制
发表时间：
2016-03-22
影响因子：
5.3
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Wu X;Ding D;Shi C;Xue Y;Zhang Z;Tang G;Tang J
玉米籽粒产量主要取决于功能叶片的光合效率，而光合效率受一系列基因网络和其他因素（包括环境条件）的控制。MicroRNAs（miRNAs）是一类在植物发育调控中起重要作用的小分子RNA。一些衰老相关的miRNAs（SA-miRNAs）通过调节其靶基因的表达水平，参与了叶片衰老的调控。为了阐明miRNAs在玉米叶片衰老中的作用及其分子机制，本研究以玉米保绿系豫87 -1和早叶衰老系ELS-1为材料，对候选miRNAs进行差异表达分析。从授粉后20和30天的穗叶中构建了4个小RNA文库，并通过Illumina深度测序技术进行测序。总共在两个品系中检测到81个miRNAs。其中，9个家族的16个miRNAs在ELS-1和Yu 87 -1之间差异表达。两个品系的表型和叶绿素含量分析将这16个差异表达的miRNAs鉴定为候选SA-miRNAs。本研究通过小分子RNA深度测序技术鉴定了16个ELS-1的候选SA-miRNAs。Degradome测序结果表明，这些候选SA-miRNAs可能通过其靶基因（主要是转录因子）调控叶片衰老，并可能控制叶绿素降解途径。这些结果突出了miRNAs在玉米叶片衰老过程中的调节作用。本文的在线版本（doi：10.1186/s12870-016-0755-y）包含补充材料，可供授权用户使用。
Maize grain yield depends mainly on the photosynthetic efficiency of functional leaves, which is controlled by an array of gene networks and other factors, including environmental conditions. MicroRNAs (miRNAs) are small RNA molecules that play important roles in plant developmental regulation. A few senescence-associated miRNAs (SA-miRNAs) have been identified as important participants in regulating leaf senescence by modulating the expression levels of their target genes. To elucidate miRNA roles in leaf senescence and their underlying molecular mechanisms in maize, a stay-green line, Yu87-1, and an early leaf senescence line, Early leaf senescence-1 (ELS-1), were selected as experimental materials for the differential expression of candidate miRNAs. Four small RNA libraries were constructed from ear leaves at 20 and 30 days after pollination and sequenced by Illumina deep sequencing technology. Altogether, 81 miRNAs were detected in both lines. Of these, 16 miRNAs of nine families were differentially expressed between ELS-1 andYu87-1. The phenotypic and chlorophyll content analyses of both lines identified these 16 differentially expressed miRNAs as candidate SA-miRNAs. In this study, 16 candidate SA-miRNAs of ELS-1 were identified through small RNA deep sequencing technology. Degradome sequencing results indicated that these candidate SA-miRNAs may regulate leaf senescence through their target genes, mainly transcription factors, and potentially control chlorophyll degradation pathways. The results highlight the regulatory roles of miRNAs during leaf senescence in maize. The online version of this article (doi:10.1186/s12870-016-0755-y) contains supplementary material, which is available to authorized users.