EPITHELIAL BASEMENT-MEMBRANE IN ALKALI-BURNED CORNEAS IN RATS - IMMUNOHISTOCHEMICAL STUDY
EPITHELIAL BASEMENT-MEMBRANE IN ALKALI-BURNED CORNEAS IN RATS - IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1097/00003226-199309000-00003
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发表时间：
1993-09-01
期刊：
影响因子：
2.8
通讯作者：
YAMANAKA, O
中科院分区：
文献类型：
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作者：
SAIKA, S;KOBATA, S;YAMANAKA, O
我们研究了角膜碱烧伤大鼠上皮基底膜（BM）成分的免疫定位改变。IV型胶原蛋白，层粘连蛋白和纤维连接蛋白在碱烧伤角膜后的不同时间间隔进行化学染色。线性层粘连蛋白免疫反应，代表BM的本地化，中断，可能是上皮酶，再生上皮24小时后。在第7天恢复正常构型，此时中央角膜BM显示在正常角膜BM中未检测到的IV型胶原免疫反应性。尚未确定愈合BM中IV型胶原免疫反应性发展的确切原因。层粘连蛋白、IV型胶原和纤连蛋白在正常基质中未检测到，但在愈合的角膜基质中均检测到了再生的角膜基质细胞。此外，再生上皮下的BM区在第5-14天显示纤维连接蛋白免疫反应性。烧伤后2个月，这些成分的分布基本正常。BM组分的改变被认为是角膜碱烧伤愈合过程的重要标志。
We investigated the altered immunolocalization of the components of epithelial basement membrane (BM) in rats with alkali burns of the cornea. Type IV collagen, laminin, and fibronectin were immunohistochemically stained in the alkali-burned corneas after various intervals. Linear laminin immunoreactivity, which represented the localization of the BM, was interrupted, probably by epithelial enzymes, under the regenerating epithelium after 24 h. A normal configuration was restored on day 7, when the central corneal BM showed a type IV collagen immunoreactivity that was not detected in BM of normal cornea. The exact cause of the development of type IV collagen immunoreactivity in the healing BM was not determined. Laminin, type IV collagen, and fibronectin, which were not detected in normal stroma, were all detected in the healing corneal stroma with repopulated keratocytes. Moreover, the BM zone under the regenerating epithelium showed fibronectin immunoreactivity on days 5-14. Distribution of these components of BM seemed to be normal 2 months postburn. The alteration of BM components is thought to be an important marker of the healing process in corneas burned with alkali.